Design of a multiplex PCR method for detection of toxigenic-pathogenic in Vibrio cholerae.

Abstract

OBJECTIVE:

To study virulence and regulatory genes (hlyA, ctxB, tcpI) in clinical strains of Vibrio cholerae (V. cholerae), simultaneously.

METHODS:

Three important genes, tcpI, hlyA and ctxB were used for detection of toxigenic and pathogenic V. cholera by chain reaction assay method.

RESULTS:

According to the results of the PCR, the incidence of hlyA, tcpI, and ctxB genes in clinical isolates was obtained as 94.7% (72 sample), 90.8% (69 sample), and 92.1% (70 sample), respectively. Five strains possessed all genes except ctxB, six strains possessed all genes except tcpI, four strains possessed all genes except hlyA, one strain possessed only hlyA and 60 strains contained a combination of three genes, Including hlyA, ctxB and tcpI.

CONCLUSIONS:

Result show that this method could be reliable to detect toxigenic-pathogenic strains of V. cholerae in Iran.

• Authors: Fooladi A A Imani, Islamieh D Iman, Doust R Hosseini, A Karami, S M Marashi
• Journal: Asian Pacific journal of tropical medicine (Asian Pac J Trop Med) Vol. 6 Issue 2 Pg. 115-8 (Feb 2013) ISSN: 2352-4146 [Electronic] India
• PMID: 23339912 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved.

Chemical References

• Bacterial Proteins
• DNA, Bacterial
• Hemolysin Proteins
• Repressor Proteins
• TcpI protein, Vibrio cholerae
• hlyA protein, Vibrio cholerae

Topics

• Bacterial Proteins (genetics)
• Cholera (microbiology)
• DNA, Bacterial (analysis, genetics)
• Feces (microbiology)
• Genes, Bacterial (genetics)
• Hemolysin Proteins (genetics)
• Humans
• Iran
• Multiplex Polymerase Chain Reaction (methods)
• Repressor Proteins (genetics)
• Sensitivity and Specificity
• Vibrio cholerae (classification, genetics, isolation & purification)
• Virulence (genetics)