Prostate cancer cells metabolize d-lactate inside mitochondria via a D-lactate dehydrogenase which is more active and highly expressed than in normal cells.

Abstract

Although D-lactate metabolism has been shown to occur in a variety of mitochondria, the metabolic fate of D-lactate in cancer cells has never been investigated, as it is believed to be exported to the extracellular phase. We show that mitochondria from both cancer (PC-3) and normal (PNT1A) prostate cells can metabolize D-lactate in an energy competent manner. This is due to the mitochondrial D-lactate dehydrogenase, a membrane flavoprotein, the activity and protein level of which are higher in PC-3 than in PNT1A cells, as detected by both kinetic and immunological analysis. D-Lactate can enter prostate mitochondria and cause the export of newly synthesized malate in a carrier-mediated manner, with the rate of malate efflux from mitochondria twofold higher in cancer.

• Authors: Lidia de Bari, Loredana Moro, Salvatore Passarella
• Journal: FEBS letters (FEBS Lett) Vol. 587 Issue 5 Pg. 467-73 (Mar 01 2013) ISSN: 1873-3468 [Electronic] England
• PMID: 23333299 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Chemical References

• Lactic Acid
• Lactate Dehydrogenases
• D-lactate dehydrogenase

Topics

• Cell Line, Tumor
• Cell Proliferation
• Cell Survival
• Humans
• Kinetics
• Lactate Dehydrogenases (metabolism)
• Lactic Acid (metabolism)
• Male
• Membrane Potential, Mitochondrial
• Mitochondria (enzymology, metabolism, physiology)
• Mitochondrial Membranes (enzymology)
• Mitochondrial Swelling
• Oxidation-Reduction
• Prostate (enzymology, pathology)
• Prostatic Neoplasms
• Protein Transport