Possible role of apoptosis in the pathogenesis and clinical evolution of radicular cyst: an immunohistochemical study.

Abstract

AIM:

To investigate whether the apoptotic cascade is activated through the extrinsic pathway in epithelial lining and connective tissue of radicular cysts.

METHODOLOGY:

Fifteen radicular cysts were fixed in formalin, embedded in paraffin wax and processed for immunohistochemistry to evaluate the expression of polyclonal antibodies against Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL), DR5 and caspase-3. Immunocomplexes were treated with the secondary antibodies and finally detected using the avidin-biotin-peroxidase complex. Immunoreactivity was visualized by development with 3,3'-diaminobenzidine. Data were analysed using the Mann-Whitney U-test; P < 0.05 was considered significant.

RESULTS:

The three antibodies were detected in connective tissue fibroblasts of all radicular cysts; TRAIL and DR5 immunoexpression was significantly greater (P < 0.05) compared with that of caspase-3. The three antibodies were also expressed in almost all epithelial layers and in endothelial cells of newly formed vessels.

CONCLUSION:

The involvement of apoptosis in the pathogenesis of radicular cysts, demonstrated by the immunoexpression patterns of TRAIL, DR5 and caspase-3 in lining epithelium and connective tissue, may explain their bland clinical aggressiveness and slow, benign evolution.

• Authors: C Loreto, C Galanti, R Leonardi, G Musumeci, G Pannone, G Palazzo, M C Rusu
• Journal: International endodontic journal (Int Endod J) Vol. 46 Issue 7 Pg. 642-8 (Jul 2013) ISSN: 1365-2591 [Electronic] England
• PMID: 23331055 (Publication Type: Journal Article)
• Copyright: © 2012 International Endodontic Journal. Published by John Wiley & Sons Ltd.

Chemical References

• Antigen-Antibody Complex
• Coloring Agents
• Receptors, TNF-Related Apoptosis-Inducing Ligand
• TNF-Related Apoptosis-Inducing Ligand
• TNFSF10 protein, human
• 3,3'-Diaminobenzidine
• Caspase 3

Topics

• 3,3'-Diaminobenzidine
• Antigen-Antibody Complex
• Apoptosis (physiology)
• Caspase 3 (analysis)
• Cell Count
• Coloring Agents
• Connective Tissue (pathology)
• Connective Tissue Cells (pathology)
• Endothelial Cells (pathology)
• Endothelium, Vascular (pathology)
• Epithelial Cells (pathology)
• Female
• Fibroblasts (pathology)
• Humans
• Immunohistochemistry
• Male
• Radicular Cyst (etiology, pathology)
• Receptors, TNF-Related Apoptosis-Inducing Ligand (analysis)
• TNF-Related Apoptosis-Inducing Ligand (analysis)