Abstract |
The pentaspan membrane glycoprotein CD133 (also known as prominin-1) has been widely used as a marker for both cancer and normal stem cells. However, the function of CD133 has not been elucidated. Here we describe a cancer stem cell line established from clear cell carcinoma of the ovary (CCC) and show that CD133 interacts with plakoglobin (also known as γ- catenin), a desmosomal linker protein. We further demonstrate that knockdown of CD133 by RNA interference (RNAi) results in the downregulation of desmoglein-2, a desmosomal cadherin, and abrogates cell-cell adhesion and tumorigenicity of CCC stem cells. We speculate that CD133 may be a promising target for cancer chemotherapy.
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Authors | Ryo Koyama-Nasu, Rina Takahashi, Satoshi Yanagida, Yukiko Nasu-Nishimura, Masaaki Oyama, Hiroko Kozuka-Hata, Ryo Haruta, Emi Manabe, Akemi Hoshino-Okubo, Hiroko Omi, Nozomu Yanaihara, Aikou Okamoto, Tadao Tanaka, Tetsu Akiyama |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 1
Pg. e53710
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 23326490
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- AC133 Antigen
- Antigens, CD
- Biomarkers, Tumor
- DSG2 protein, human
- Desmoglein 2
- Glycoproteins
- PROM1 protein, human
- Peptides
- Prom1 protein, mouse
- gamma Catenin
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Topics |
- AC133 Antigen
- Animals
- Antigens, CD
(metabolism)
- Biomarkers, Tumor
(metabolism)
- Caco-2 Cells
- Cell Adhesion
- Cell Transformation, Neoplastic
(metabolism, pathology)
- Desmoglein 2
(metabolism)
- Glycoproteins
(metabolism)
- Humans
- Mice
- Neoplastic Stem Cells
(metabolism, pathology)
- Peptides
(metabolism)
- Protein Binding
- gamma Catenin
(metabolism)
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