Assessment of neutrophil gelatinase-associated lipocalin and lactate dehydrogenase in peritoneal fluids for the screening of bacterial peritonitis.

Abstract

BACKGROUND:

Manual microscopy remains the gold standard for enumeration and classification of nucleated cells in peritoneal fluids, especially for diagnosing bacterial peritonitis. However, this approach carries several drawbacks, so that the use of simple and automated tests may be a viable option for initial screening of peritoneal fluids.

MATERIALS AND METHODS:

Neutrophil gelatinase-associated lipocalin (NGAL), lactate dehydrogenase (LDH), proteins and glucose were assessed in peritoneal fluids from patients with new onset nonmalignant ascites, along with nucleated cell count and differentiation.

RESULTS:

One hundred and eleven specimens were analyzed, 26 of which (23%) with polymorphonuclear leukocyte (PMN) count≥250/μL, thus compatible with bacterial peritonitis. The median concentration of LDH and NGAL was 3.4 and 3.7-fold higher in samples with ≥250 PMN/μL. The concentration of proteins was also higher in samples with ≥250 PMN/μL, whereas that of glucose was lower. The PMN count significantly correlated with peritoneal fluid values of LDH (r=0.859), NGAL (r=0.774) and proteins (r=0.268), but not with glucose (r=-0.069). The area under ROC curve was 0.88 for LDH, 0.89 for NGAL and 0.94 for their combination (both tests positive), whereas that of proteins and glucose was 0.80 and 0.71, respectively. Sensitivities and specificities were 0.81 and 0.87 for LDH≥227 U/L, 0.96 and 0.75 for NGAL≥120 ng/mL, 0.77 and 0.95 for their combination. The agreement with PMN count was 0.86 for LDH, 0.80 for NGAL, and 0.91 for their combination.

CONCLUSIONS:

These results suggest that assessment of NGAL in peritoneal fluids, especially in combination with LDH, may be a reliable approach for screening of bacterial peritonitis in patients with new onset nonmalignant ascites.

• Authors: Giuseppe Lippi, Alberta Caleffi, Silvia Pipitone, Gianfranco Elia, Anna Ngah, Rosalia Aloe, Paola Avanzini, Carlo Ferrari
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 418 Pg. 59-62 (Mar 15 2013) ISSN: 1873-3492 [Electronic] Netherlands
• PMID: 23318563 (Publication Type: Journal Article)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Acute-Phase Proteins
• LCN2 protein, human
• Lipocalin-2
• Lipocalins
• Proto-Oncogene Proteins
• L-Lactate Dehydrogenase

Topics

• Acute-Phase Proteins (analysis, metabolism)
• Ascitic Fluid (enzymology)
• Humans
• L-Lactate Dehydrogenase (analysis, metabolism)
• Leukocyte Count
• Lipocalin-2
• Lipocalins (analysis, metabolism)
• Peritonitis (diagnosis, metabolism)
• Proto-Oncogene Proteins (analysis, metabolism)
• ROC Curve
• Risk Assessment
• Sensitivity and Specificity