Rapid determination of isoamyl nitrite in pharmaceutical preparations by flow injection analysis with on-line UV irradiation and luminol chemiluminescence detection.

Abstract

Isoamyl nitrite is used as a therapeutic reagent for cardiac angina and as an antidote for cyanide poisoning, but it is abused because of its euphoric properties. Therefore, a method to determine isoamyl nitrite is required in many fields, including pharmaceutical and forensic studies. In this study, a simple, rapid and sensitive method for the determination of isoamyl nitrite was developed using a flow injection analysis system equipped with a chemiluminescence detector and on-line photoreactor. This method is based on on-line ultraviolet irradiation of isoamyl nitrite and subsequent luminol chemiluminescence detection without the addition of an oxidant. A linear standard curve was obtained up to 1.0 μM of isoamyl nitrite with a detection limit (blank + 3SD) of 0.03 μM. The method was successfully applied to determine isoamyl nitrite content in pharmaceutical preparations.

• Authors: Naoya Kishikawa, Naoko Kondo, Abena Amponsaa-Karikari, Hitoshi Kodamatani, Kaname Ohyama, Kenichiro Nakashima, Shigeo Yamazaki, Naotaka Kuroda
• Journal: Luminescence : the journal of biological and chemical luminescence (Luminescence) Vol. 29 Issue 1 Pg. 8-12 (Feb 2014) ISSN: 1522-7243 [Electronic] England
• PMID: 23299968 (Publication Type: Journal Article, Validation Study)
• Copyright: Copyright © 2013 John Wiley & Sons, Ltd.

Chemical References

• Pharmaceutical Preparations
• Luminol
• isoamyl nitrite
• Amyl Nitrite

Topics

• Amyl Nitrite (analogs & derivatives, analysis)
• Calibration
• Luminescence
• Luminol (chemistry)
• Pharmaceutical Preparations (chemistry)
• Ultraviolet Rays