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Syndecan-2 is a key regulator of transforming growth factor beta 2/Smad2-mediated adhesion in fibrosarcoma cells.

Abstract
Fibrosarcoma is a rare malignant tumor originating from fibroblasts. Transforming growth factor beta 2 (TGFβ2) has been established to regulate processes correlated to fibrosarcoma tumorigenesis. In this study, we investigated the possible participation of syndecan-2 (SDC-2), a cell membrane heparan sulfate (HS) proteoglycan on these TGFβ2 functions. Our results demonstrate that the inhibition of SDC-2 expression by short interfering RNA (siSDC2) abolished TGFβ2-dependent HT1080 cell adhesion (P ≤ 0.01). In parallel, the downregulation of SDC-2 significantly inhibited TGFβ2-induced Smad2 phosphorylation (P ≤ 0.01). The immunoflourescence signal of TGF receptor III as well as its protein expression was decreased in SDC-2-deficient cells. The enhancement of adhesion molecules integrin β1 (P ≤ 0.01) and focal adhesion kinase expression, induced by TGFβ2 treatment (P ≤ 0.001), was markedly inhibited in SDC-2-defficient cells (P ≤ 0.01). Conclusively, the obtained data suggest that SDC-2 modulates TGFβ2 transcriptional regulation via Smad signaling to facilitate fibrosarcoma cell adhesion.
AuthorsMaria Mytilinaiou, Artan Bano, Dragana Nikitovic, Aikaterini Berdiaki, Kallirroi Voudouri, Alexandra Kalogeraki, Nikos K Karamanos, George N Tzanakakis
JournalIUBMB life (IUBMB Life) Vol. 65 Issue 2 Pg. 134-43 (Feb 2013) ISSN: 1521-6551 [Electronic] England
PMID23297089 (Publication Type: Journal Article)
CopyrightCopyright © 2013 International Union of Biochemistry and Molecular Biology, Inc.
Chemical References
  • Actins
  • Fibronectins
  • Integrin beta1
  • RNA, Small Interfering
  • Receptors, Transforming Growth Factor beta
  • SDC2 protein, human
  • SMAD2 protein, human
  • Smad2 Protein
  • Transforming Growth Factor beta2
  • Syndecan-2
Topics
  • Actins (metabolism)
  • Cell Adhesion
  • Cell Line, Tumor
  • Fibronectins (metabolism)
  • Fibrosarcoma
  • Gene Expression
  • Gene Knockdown Techniques
  • Humans
  • Integrin beta1 (genetics, metabolism)
  • Protein Multimerization
  • Protein Transport
  • RNA, Small Interfering (genetics)
  • Receptors, Transforming Growth Factor beta (genetics, metabolism)
  • Smad2 Protein (metabolism)
  • Syndecan-2 (genetics, metabolism)
  • Transforming Growth Factor beta2 (physiology)

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