Identification and characterization of two peptidoglycan recognition proteins with zinc-dependent antibacterial activity from the cotton bollworm, Helicoverpa armigera.

Abstract

Peptidoglycan recognition proteins (PGRPs) specifically bind to peptidoglycan and play an important role in the innate immune responses as pattern recognition receptors (PRRs). Here we identified and characterized two PGRPs (HaPGRP-B and HaPGRP-C) from the cotton bollworm, Helicoverpa armigera. The comparative analysis indicated that five amino acids which are required for T7 lysozyme Zn(2+) binding and amidase activity are conserved in HaPGRP-B and HaPGRP-C, suggesting that the two PGRPs are members of the amidase-type PGRPs. HaPGRP-B and HaPGRP-C mRNA increased in both the fat bodies and the hemocytes after an injection of Gram-negative Escherichia coli or Gram-positive Staphylococcus aureus. Recombinant HaPGRP-B and HaPGRP-C could agglutinate E. coli and S. aureus in a zinc-dependent manner. More importantly, both rHaPGRP-B and rHaPGRP strongly inhibited the growth of E. coli and S. aureus in the presence of Zn(2+). Moreover, the HaPGRP-B mRNA showed up-regulation post hormones (20E and methoprene) injection. Our results indicate that the two PGRPs of H. armigera may play an important role in defending against bacteria as amidase-type PGRPs and the hormones can function in regulating the expressions of PGRPs.

• Authors: Dai-Qun Yang, Zhen-Lin Su, Chuan Qiao, Zan Zhang, Jia-Lin Wang, Fei Li, Xu-Sheng Liu
• Journal: Developmental and comparative immunology (Dev Comp Immunol) Vol. 39 Issue 4 Pg. 343-51 (Apr 2013) ISSN: 1879-0089 [Electronic] United States
• PMID: 23295246 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Carrier Proteins
• RNA, Messenger
• Recombinant Proteins
• peptidoglycan recognition protein
• Amidohydrolases
• N-Acetylmuramoyl-L-alanine Amidase
• amidase
• Zinc

Topics

• Amidohydrolases (metabolism)
• Amino Acid Sequence
• Animals
• Anti-Bacterial Agents (metabolism)
• Base Sequence
• Carrier Proteins (chemistry, genetics, immunology, metabolism)
• Escherichia coli (drug effects)
• Immunity, Innate
• Microbial Sensitivity Tests
• Moths (metabolism)
• N-Acetylmuramoyl-L-alanine Amidase (metabolism)
• RNA, Messenger (genetics)
• Recombinant Proteins
• Sequence Alignment
• Sequence Analysis, DNA
• Staphylococcus aureus
• Zinc (metabolism)