Knockdown of Coronin-1C disrupts Rac1 activation and impairs tumorigenic potential in hepatocellular carcinoma cells.

Abstract	Coronin-1C is an important F-actin binding protein which is critical for cell motility. Furthermore, the expression of this protein was found to be increased in diffuse tumors and was correlated with the degree of tumor malignancy. However, the mechanism(s) through which this protein enhances malignancy in hepatocellular carcinoma (HCC) is poorly understood. In this study, we found that Coronin-1C was overexpressed in human HCC tissues compared with the adjacent non-tumor tissues. Overexpression of Coronin-1C enhanced the cell migration in the human HCC cell line BEL-7402, whereas suppressed cell migration and proliferation were observed in Coronin-1C-knockdown BEL-7402 cells together with impaired cell polarity, disrupted cytoskeleton and decreased Rac-1 activation. Moreover, the Coronin-1C knockdown cells displayed a lower degree of malignancy by inducing smaller tumors in nude mice. Thus, we demonstrated a relationship between Coronin-1C overexpression and human HCC growth through enhancement of tumor cell proliferation and migration, which are correlated with Rac-1 activation.
Authors	Zhi-Gang Wang, Ming-Ku Jia, Hong Cao, Peng Bian, Xue-Dong Fang
Journal	Oncology reports (Oncol Rep) Vol. 29 Issue 3 Pg. 1066-72 (Mar 2013) ISSN: 1791-2431 [Electronic] Greece
PMID	23292607 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Microfilament Proteins RAC1 protein, human coronin proteins rac1 GTP-Binding Protein
Topics	Aged Animals Carcinogenesis (metabolism) Carcinoma, Hepatocellular (metabolism, pathology) Cell Line, Tumor Cell Movement Cell Polarity Cell Proliferation Cytoskeleton (metabolism) Enzyme Activation Female Gene Knockdown Techniques Humans Liver Neoplasms (metabolism, pathology) Male Mice Mice, Nude Microfilament Proteins (genetics, metabolism) Middle Aged Neoplasm Invasiveness Neoplasm Transplantation Tumor Burden rac1 GTP-Binding Protein (metabolism)

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