Confocal microscopy study of pertussis toxin and toxoids on CHO-cells.

Abstract

Pertussis toxin in its detoxified form is a major component of all current acellular pertussis vaccines. Here we report the membrane translocation and internalization activities of pertussis toxin and various pertussis toxoids using Chinese hamster ovary cells and confocal microscopy based on indirect immunofluorescence labeling. Chemically detoxified pertussis toxoids were able to translocate/internalize into cells at the concentration about 1,000 times higher than the native toxin. Pertussis toxoids detoxified with different procedures (glutaraldehyde, glutaraldehyde plus formaldehyde, hydrogen peroxide or genetic mutation) showed differences in fluorescence intensity under the same condition, indicating toxoids from different detoxification methods may have different translocation/internalization activities on cells.

• Authors: Yajun Tan, Roland A Fleck, Catpagavalli Asokanathan, Chun-Ting Yuen, Dorothy Xing, Shumin Zhang, Junzhi Wang
• Journal: Human vaccines & immunotherapeutics (Hum Vaccin Immunother) Vol. 9 Issue 2 Pg. 332-8 (Feb 2013) ISSN: 2164-554X [Electronic] United States
• PMID: 23291938 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Toxoids
• Pertussis Toxin

Topics

• Animals
• CHO Cells
• Cricetulus
• Microscopy, Confocal
• Pertussis Toxin (metabolism)
• Protein Transport
• Toxoids (metabolism)