Abstract |
A membrane protein of M. W. 32,000 D and pI 5 has been purified from EDTA-treated plasma membrane supernatants by Page without detergents. These membranes are purified from the MF2S cell line issued from the murine plasmacytoma MOPC 173. The purified protein was able to induce a 300 fold increase in the Na+/K+ ATPase resistance to ouabaïn thus mimicking the original resistance of the enzyme to the drug.
|
Authors | B Geny, D Charlemagne, A Paraf |
Journal | Comptes rendus des seances de l'Academie des sciences. Serie D, Sciences naturelles
(C R Seances Acad Sci D)
Vol. 289
Issue 14
Pg. 1057-60
(Nov 26 1979)
ISSN: 0567-655X [Print] France |
Vernacular Title | Purification d'une protéine membranaire modifiant la sensibilité de l'ATPase Na+/K+ à l'ouabaïne à partir du surnageant de membranes plasmiques traitées à l'EDTA. |
PMID | 232860
(Publication Type: Journal Article)
|
Chemical References |
- Membrane Proteins
- Ouabain
- Edetic Acid
- Sodium-Potassium-Exchanging ATPase
|
Topics |
- Animals
- Cell Line
- Cell Membrane
(physiology)
- Edetic Acid
(pharmacology)
- Kinetics
- Membrane Proteins
(isolation & purification, physiology)
- Mice
- Molecular Weight
- Ouabain
(pharmacology)
- Plasmacytoma
- Sodium-Potassium-Exchanging ATPase
(metabolism)
|