Abstract | OBJECTIVE: METHODS: RESULTS: Analysis of the sera of mice immunized with citrullinated proteins revealed false-positive results with the citrulline peptide-based ELISA. In contrast, Western blot analysis using either citrullinated or native fibrinogen as substrate reliably detected ACPAs in Balb/c mice immunized with citrullinated fibrinogen, MBP, and CII. However, these ACPAs failed to induce or aggravate disease in Balb/c mice in the anticollagen antibody-induced arthritis model. Immunization with citrullinated fibrinogen induced ACPAs but did not lead to arthritis development in SJL and DBA/1 mice. In contrast, immunization with citrullinated CII failed to induce ACPAs or enhance disease in these strains in the collagen-induced arthritis model. CONCLUSION: Mice can develop genuine ACPAs, but detection of ACPAs is highly dependent on strain, immunogen, immunization protocol, and detection assay. Murine ACPAs are not overtly pathogenic, since neither preexisting ACPAs nor the use of citrullinated collagen as immunogen modulates the clinical course of arthritis.
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Authors | Tineke Cantaert, Christine Teitsma, Paul P Tak, Dominique Baeten |
Journal | Arthritis and rheumatism
(Arthritis Rheum)
Vol. 65
Issue 4
Pg. 939-48
(Apr 2013)
ISSN: 1529-0131 [Electronic] United States |
PMID | 23280233
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 by the American College of Rheumatology. |
Chemical References |
- Adjuvants, Immunologic
- Autoantibodies
- Autoantigens
- Collagen Type II
- Mbp protein, mouse
- Myelin Basic Protein
- Proteins
- Citrulline
- Fibrinogen
- Freund's Adjuvant
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Topics |
- Adjuvants, Immunologic
- Animals
- Arthritis, Experimental
(immunology, therapy)
- Arthritis, Rheumatoid
(immunology, therapy)
- Autoantibodies
(genetics, immunology)
- Autoantigens
(immunology)
- Citrulline
- Collagen Type II
(immunology)
- Disease Models, Animal
- Enzyme-Linked Immunosorbent Assay
- False Positive Reactions
- Fibrinogen
(immunology)
- Freund's Adjuvant
(immunology)
- Genetic Predisposition to Disease
- Immunotherapy, Active
- Mice
- Mice, Inbred BALB C
- Mice, Inbred DBA
- Myelin Basic Protein
(immunology)
- Protein Processing, Post-Translational
- Proteins
(immunology)
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