Abstract | BACKGROUND AND PURPOSE: The transient receptor potential vanilloid type 1 (TRPV1) plays a fundamental role in the detection of heat and inflammatory pain responses. Here we investigated the contribution of two potential endogenous ligands [9- and 13- hydroxyoctadecadienoic acid (HODE)] to TRPV1-mediated noxious responses and inflammatory pain responses. EXPERIMENTAL APPROACH: 9- and 13-HODE, and their precursor, linoleic acid, were measured in dorsal root ganglion (DRG) neurons and in the hindpaws of control and carrageenan-inflamed rats by liquid chromatography/tandem electrospray mass spectrometry. Calcium imaging studies of DRG neurons were employed to determine the role of TRPV1 in mediating linoleic acid, 9-HODE- and 13-HODE-evoked responses, and the contribution of 15-lipoxygenase to the generation of the HODEs. Behavioural studies investigated the contribution of 9- and 13-HODE and 15-lipoxygenase to inflammatory pain behaviour. KEY RESULTS:
9-HODE (35 ± 7 pmol g(-1)) and 13-HODE (32 ± 6 pmol g(-1)) were detected in hindpaw tissue, but were below the limits of detection in DRGs. Following exposure to linoleic acid, 9- and 13-HODE were detected in DRGs and TRPV1 antagonist-sensitive calcium responses evoked, which were blocked by the 15-lipoxygenase inhibitor PD146176 and an anti-13-HODE antibody. Levels of linoleic acid were significantly increased in the carrageenan-inflamed hindpaw (P < 0.05), whereas levels of 9- and 13-HODE were, however, decreased. Intraplantar co-administration of anti-9- and 13-HODE antibodies and treatment with PD146176 significantly (P < 0.01) attenuated carrageenan-induced hyperalgesia. CONCLUSIONS AND IMPLICATIONS: This study demonstrates that, although 9- and 13-HODE can activate TRPV1 in DRG cell bodies, the evidence for a role of these lipids as endogenous peripheral TRPV1 ligands in a model of inflammatory pain is at best equivocal.
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Authors | Mohammad Alsalem, Amy Wong, Paul Millns, Pallavi Huma Arya, Michael Siang Liang Chan, Andrew Bennett, David A Barrett, Victoria Chapman, David A Kendall |
Journal | British journal of pharmacology
(Br J Pharmacol)
Vol. 168
Issue 8
Pg. 1961-74
(Apr 2013)
ISSN: 1476-5381 [Electronic] England |
PMID | 23278358
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2012 The Authors. British Journal of Pharmacology © 2012 The British Pharmacological Society. |
Chemical References |
- 6,11-dihydro-5-thia-11-aza-benzo(a)-fluorene
- Fluorenes
- Linoleic Acids
- Linoleic Acids, Conjugated
- TRPV Cation Channels
- TRPV1 protein, mouse
- 9-hydroxy-10,12-octadecadienoic acid
- 13-hydroxy-9,11-octadecadienoic acid
- Arachidonate 15-Lipoxygenase
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Topics |
- Animals
- Arachidonate 15-Lipoxygenase
(metabolism)
- Disease Models, Animal
- Fluorenes
(pharmacology)
- Ganglia, Spinal
(metabolism)
- Inflammation
(chemically induced, complications, drug therapy, metabolism)
- Linoleic Acids
(metabolism, pharmacology)
- Linoleic Acids, Conjugated
(metabolism)
- Male
- Mice
- Pain
(complications, drug therapy, metabolism)
- Rats, Sprague-Dawley
- TRPV Cation Channels
(metabolism)
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