Immunoexpression of RANK, RANKL, OPG, VEGF, and vWF in radicular and dentigerous cysts.

Abstract

BACKGROUND:

Radicular (RC) and dentigerous cysts (DC) can show a range from little to quite extensive primary/secondary inflammation and it is possible that the variation seen in the fibrous capsule of these cysts might reflect differences in the osteolytic activity. Moreover, the presence of hemorrhagic areas in the fibrous capsule of DC could also contribute to the increase in osteolytic activity. The aim of this study was to compare immunohistochemical expression of nuclear factor κappaB (RANK), RANK ligand (RANKL), and osteoprotegerin (OPG), vascular endothelial growth factor (VEGF) and angiogenic index in RC and DC.

METHODS:

These proteins were evaluated in 20 RC and DC by immunohistochemistry. Angiogenic index was determined by microvessel count (MVC) using anti-von Willebrand factor antibody.

RESULTS:

RANK and RANKL were higher in DC than RC in fibrous capsule. RC showed higher expression of VEGF in the epithelium and capsule. DC exhibited higher MVC than RC.

CONCLUSIONS:

Ours results suggest that RANK and RANKL play an important role in bone resorption in DC and the hemorrhagic areas in the capsule of DC could be explained by increased vessel's number. The higher VEGF expression in RC might be related to nature of these lesions, where the inflammatory process contributes significantly to these findings.

• Authors: Maiara de Moraes, Felipe Rodrigues de Matos, Lélia Batista de Souza, Roseana de Almeida Freitas, Antônio de Lisboa Lopes Costa
• Journal: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (J Oral Pathol Med) Vol. 42 Issue 6 Pg. 468-73 (Jul 2013) ISSN: 1600-0714 [Electronic] Denmark
• PMID: 23278112 (Publication Type: Comparative Study, Journal Article)
• Copyright: © 2012 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• Osteoprotegerin
• RANK Ligand
• Receptor Activator of Nuclear Factor-kappa B
• TNFRSF11A protein, human
• TNFRSF11B protein, human
• TNFSF11 protein, human
• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• von Willebrand Factor

Topics

• Bone Resorption (pathology)
• Cell Count
• Cell Nucleus (pathology)
• Connective Tissue (pathology)
• Cytoplasm (pathology)
• Dentigerous Cyst (pathology)
• Epithelial Cells (pathology)
• Epithelium (pathology)
• Humans
• Immunohistochemistry
• Microvessels (pathology)
• Neovascularization, Pathologic (pathology)
• Osteoprotegerin (analysis)
• RANK Ligand (analysis)
• Radicular Cyst (pathology)
• Receptor Activator of Nuclear Factor-kappa B (analysis)
• Stromal Cells (pathology)
• Vascular Endothelial Growth Factor A (analysis)
• von Willebrand Factor (analysis)