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Influenza A virus nucleoprotein derived from Escherichia coli or recombinant vaccinia (Tiantan) virus elicits robust cross-protection in mice.

AbstractBACKGROUND:
Immunity to conserved viral antigens is an attractive approach to develop a universal vaccine against epidemic and pandemic influenza. A nucleoprotein (NP)-based vaccine has been explored and preliminary studies have shown promise. However, no study has explored the immunity and cross-protective efficacy of recombinant NP derived from Escherichia coli compared with recombinant vaccinia virus (Tiantan).
METHODS:
Recombinant NP protein (rNP) from influenza virus A/Jingke/30/95(H3N2) was obtained from E. coli and recombinant vaccinia virus (Tiantan) RVJ1175NP. Purified rNP without adjuvant and RVJ1175NP were used to immunize BALB/c mice intramuscularly. Humoral immune responses were detected by ELISA, while cell-mediated immune responses were measured by ex vivo IFN-γ ELISPOT and in vivo cytotoxicity assays. The cross-protective efficacy was assessed by a challenge with a heterosubtype of influenza virus A/PR/8/34(H1N1).
RESULTS:
Our results demonstrate that a high dose (90 μg) of rNP induced NP-specific antibodies and T cell responses that were comparable with those of RVJ1175NP in mice. Importantly, the survival ratio (36, 73, and 78%) of the vaccinated mice after the influenza virus A/PR/8/34(H1N1) challenge was rNP vaccine dose-dependent (10, 30, and 90 μg, respectively), and no significant differences were observed between the rNP- and RVJ1175NP-immunized (91%) mice.
CONCLUSIONS:
Influenza A virus NP derived from E. coli or recombinant vaccinia (Tiantan) virus elicited cross-protection against influenza virus in mice, and the immune response and protective efficacy of rNP were comparable to RVJ1175NP. These data provide a basis for the use of prokaryotically expressed NP as a candidate universal influenza vaccine.
AuthorsBaoying Huang, Wenling Wang, Renqing Li, Xiuping Wang, Tao Jiang, Xiangrong Qi, Yingying Gao, Wenjie Tan, Li Ruan
JournalVirology journal (Virol J) Vol. 9 Pg. 322 ( 2012) ISSN: 1743-422X [Electronic] England
PMID23272943 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Viral
  • Influenza Vaccines
  • NP protein, Influenza A virus
  • RNA-Binding Proteins
  • Recombinant Proteins
  • Vaccines, Synthetic
  • Viral Core Proteins
Topics
  • Animals
  • Antibodies, Viral (immunology)
  • Cross Protection
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay
  • Escherichia coli (genetics, metabolism)
  • Female
  • Immunity, Cellular
  • Immunization Schedule
  • Influenza A Virus, H1N1 Subtype (genetics, immunology, metabolism)
  • Influenza A Virus, H3N2 Subtype (genetics, immunology, metabolism)
  • Influenza Vaccines (genetics, immunology, metabolism)
  • Mice
  • Mice, Inbred BALB C
  • Orthomyxoviridae Infections (immunology, prevention & control)
  • Plasmids (genetics, metabolism)
  • RNA-Binding Proteins (genetics, immunology, metabolism)
  • Recombinant Proteins (genetics, immunology, metabolism)
  • Survival Analysis
  • Transformation, Genetic
  • Vaccination
  • Vaccines, Synthetic (immunology)
  • Vaccinia virus (genetics, metabolism)
  • Viral Core Proteins (genetics, immunology, metabolism)

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