Silencing of the ARP2/3 complex disturbs pancreatic cancer cell migration.

Abstract

BACKGROUND:

Actin-related protein 2/3 (ARP2/3) complex is an actin nucleator responsible for actin cytoskeleton branching which is essential for efficient cell migration.

MATERIALS AND METHODS:

The expression of the seven ARP2/3 complex subunits was assessed in pancreatic cancer cell lines and in normal pancreatic samples by quantitative RT-PCR. siRNA-mediated silencing was used to study the contribution of each ARP2/3 complex member to pancreatic cancer cell migration.

RESULTS:

ARPC3 and ARPC4 were the most highly expressed complex members, while ARPC1B and ARPC2 were expressed at low levels. Silencing of the ARP2/3 complex subunits typically resulted in reduced cell migration capacity. In particular, silencing of ARPC4 significantly reduced cell migration in all studied cell lines, with a major impact on Hs700T and HPAFII migration (50% and 68% decrease, p<0.001).

CONCLUSION:

We offer comprehensive expression data on the ARP2/3 complex members for pancreatic cancer and normal pancreas. In addition, we show cell line-specific differences in ARP2/3 complex subunit dependency on cell migratory potential, and suggest ARPC4 to be one of the key members of the ARP2/3 complex in pancreatic cancer.

• Authors: Hanna E Rauhala, Susanna Teppo, Sanna Niemelä, Anne Kallioniemi
• Journal: Anticancer research (Anticancer Res) Vol. 33 Issue 1 Pg. 45-52 (Jan 2013) ISSN: 1791-7530 [Electronic] Greece
• PMID: 23267127 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ARPC4 protein, human
• Actin-Related Protein 2-3 Complex
• Actins
• Cytoskeletal Proteins
• RNA, Small Interfering

Topics

• Actin Cytoskeleton (metabolism)
• Actin-Related Protein 2-3 Complex (genetics, metabolism)
• Actins (genetics, metabolism)
• Cell Line, Tumor
• Cell Movement
• Cytoskeletal Proteins (genetics, metabolism)
• Gene Expression Regulation, Neoplastic
• Humans
• Pancreatic Neoplasms (genetics, metabolism, pathology)
• RNA, Small Interfering