HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Surfactant free preparation of biodegradable dendritic polyglycerol nanogels by inverse nanoprecipitation for encapsulation and release of pharmaceutical biomacromolecules.

Abstract
In this paper we report a novel approach to generate biodegradable polyglycerol nanogels on different length scales. We developed a mild, surfactant free inverse nanoprecipitation process to template hydrophilic polyglycerol nanoparticles. In situ crosslinking of the precipitated nanoparticles by bioorthogonal copper catalyzed click chemistry allows us to obtain size defined polyglycerol nanogels (100-1000nm). Biodegradability was achieved by the introduction of benzacetal bonds into the net points of the nanogel. Interestingly, the polyglycerol nanogels quickly degraded into low molecular weight fragments at acidic pH values, which are present in inflamed and tumor tissues as well as intracellular organelles, and they remained stable at physiological pH values for a long time. This mild approach to biodegradable polyglycerol nanogels allows us to encapsulate labile biomacromolecules such as proteins, including the therapeutic relevant enzyme asparaginase, into the protein resistant polyglycerol network. Enzymes were encapsulated with an efficacy of 100% and after drug release, full enzyme activity and structural integrity were retained. This new inverse nanoprecipitation procedure allows the efficient encapsulation and release of various biomacromolecules including proteins and could find many applications in polymer therapeutics and nanomedicine.
AuthorsDirk Steinhilber, Madeleine Witting, Xuejiao Zhang, Michael Staegemann, Florian Paulus, Wolfgang Friess, Sarah Küchler, Rainer Haag
JournalJournal of controlled release : official journal of the Controlled Release Society (J Control Release) Vol. 169 Issue 3 Pg. 289-95 (Aug 10 2013) ISSN: 1873-4995 [Electronic] Netherlands
PMID23262202 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 Elsevier B.V. All rights reserved.
Chemical References
  • Delayed-Action Preparations
  • Dendrimers
  • Nanogels
  • Polymers
  • Proteins
  • polyethylene glycol polyethyleneimine nanogel
  • polyglycerol
  • Polyethylene Glycols
  • Polyethyleneimine
  • Asparaginase
  • Glycerol
Topics
  • Asparaginase (administration & dosage, metabolism)
  • Chemical Precipitation
  • Delayed-Action Preparations (chemistry)
  • Dendrimers (chemistry)
  • Glycerol (chemistry)
  • Hydrophobic and Hydrophilic Interactions
  • Models, Molecular
  • Nanogels
  • Nanoparticles (chemistry)
  • Polyethylene Glycols (chemistry)
  • Polyethyleneimine (chemistry)
  • Polymers (chemistry)
  • Proteins (administration & dosage, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: