The performance and safety of current
antineoplastic agents, particularly water-insoluble drugs, are still far from satisfactory. For example, the currently widely used
Cremophor EL®-based
paclitaxel (PTX) formulation exhibits pharmacokinetic concerns and severe side effects. Thus, the concept of a biodegradable polymeric drug-delivery system, which can significantly improve therapeutic efficacy and reduce side effects is advocated. The present work aims to develop a new-generation of long-circulating, biodegradable carriers for effective delivery of PTX. First, a multiblock backbone biodegradable N-(2-hydroxypropyl)methacrylamide(HPMA) copolymer-PTX conjugate (
mP-PTX) with molecular weight (Mw) of 335 kDa was synthesized by RAFT (reversible addition-fragmentation chain transfer) copolymerization, followed by chain extension. In vitro studies on human ovarian
carcinoma A2780 cells were carried out to investigate the cytotoxicity of free PTX,
HPMA copolymer-PTX conjugate with Mw of 48 kDa (P-PTX), and
mP-PTX. The experiments demonstrated that
mP-PTX has a similar cytotoxic effect against A2780 cells as free PTX and P-PTX. To further compare the behavior of this new biodegradable conjugate (
mP-PTX) with free PTX and P-PTX in vivo evaluation was performed using female nu/nu mice bearing orthotopic A2780 ovarian
tumors. Pharmacokinetics study showed that high Mw
mP-PTX was cleared more slowly from the blood than commercial PTX formulation and low Mw P-PTX. SPECT/CT imaging and biodistribution studies demonstrated biodegradability as well as elimination of
mP-PTX from the body. The
tumors in the
mP-PTX treated group grew more slowly than those treated with saline, free PTX, and P-PTX (single dose at 20 mg PTX/kg equivalent). Moreover, mice treated with
mP-PTX had no obvious
ascites and
body-weight loss. Histological analysis indicated that
mP-PTX had no toxicity in liver and spleen, but induced massive cell death in the
tumor. In summary, this biodegradable drug delivery system has a great potential to improve performance and safety of current
antineoplastic agents.