The protective effects of dexmedetomidine on the liver and remote organs against hepatic ischemia reperfusion injury in rats.

Abstract

AIM:

To investigate the protective effects of dexmedetomidine against hepatic ischemia/reperfusion (IR) injury and hepatic IR induced remote organ injury.

METHODS:

Forty Wistar albino rats were divided into the following four groups: sham, dexmedetomidine, IR, and IR + dexmedetomidine. Hepatic ischemia was created by the Pringle maneuver for 30 min followed by a 30 min reperfusion period in the IR and IR + dexmedetomidine groups. The dexmedetomidine and IR + dexmedetomidine groups were administered dexmedetomidine (100 μg/kg, single dose) intraperitoneally after the anesthesia insult. Blood samples and hepatic, renal, and lung tissue specimens were obtained to measure serum and tissue total oxidative activity (TOA), total antioxidant capacity (TAC), paraoxonase (PON-1), and oxidative stress index (OSI) after 60 min in all groups.

RESULTS:

According to the biochemical analyses of the samples taken from the serum and the liver, lung, and kidney tissues, when comparing the sham group and the IR group, TOA and OSI values were higher in the IR group, while TAC and PON-1 values were lower (p < 0.05). It was observed that TOA and OSI values were significantly lower, while TAC and PON-1 values increased with dexmedetomidine treatment (p < 0.05). In addition, dexmedetomidine ameliorated hepatic histopathological changes inducing IR, but there were no significant histopathological changes in the remote organs.

CONCLUSION:

This study demonstrated that dexmedetomidine markedly reduced the oxidative stress in serum, liver, and remote organs induced by hepatic IR injury, and ameliorated the histopathological damage in the liver.

• Authors: Adnan Tüfek, Orhan Tokgöz, Ibrahim Aliosmanoglu, Ulaş Alabalik, Osman Evliyaoglu, Taner Çiftçi, Abdülmenap Güzel, Zeynep Baysal Yıldırım
• Journal: International journal of surgery (London, England) (Int J Surg) Vol. 11 Issue 1 Pg. 96-100 ( 2013) ISSN: 1743-9159 [Electronic] England
• PMID: 23261946 (Publication Type: Journal Article)
• Copyright: Copyright © 2012 Surgical Associates Ltd. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Protective Agents
• Dexmedetomidine
• Aryldialkylphosphatase

Topics

• Animals
• Aryldialkylphosphatase (blood)
• Dexmedetomidine (pharmacology)
• Histocytochemistry
• Kidney (drug effects, enzymology)
• Liver (blood supply, drug effects, metabolism, pathology)
• Lung (drug effects, enzymology)
• Male
• Oxidative Stress
• Protective Agents (pharmacology)
• Rats
• Rats, Wistar
• Reperfusion Injury (blood, prevention & control)
• Statistics, Nonparametric