[Basic and clinical evaluation of measurement of pancreatic cancer associated antigen, SPan-1].

Abstract

Basic evaluation of SPan-1 assay (SPan-1 RIA. BEAD) and clinical significance of serum SPan-1 levels for the diagnosis of pancreatic cancer were studied. This assay was reproducible, reliable and simple to perform. It required minimal samples (duplicate 50 microliters) and may be done within 4 hrs. Normal subjects (N = 1182) had serum SPan-1 antigen levels which ranged 0 to 42.8 units/ml with a mean of 7.5 units/ml and above 40 units/ml was considered to be positive. SPan-1 antigen levels in cultured medium of four out of five pancreatic cancer cell lines showed more than 1000 units/ml by this assay. While over 90% of pancreatic cancer patients had elevated levels of serum SPan-1 antigen, only 0-17% of patients with other malignant and non malignant gastrointestinal diseases such as pancreatitis (chronic or acute), gastric cancer or colon cancer had above normal levels. Furthermore, levels of serum SPan-1 antigen correlated well with treatment and recurrence of disease in patients with pancreatic and gastric cancer. These results suggest that determination of serum SPan-1 antigen levels by this assay kit is useful for the diagnosis and monitoring of pancreatic cancer.

• Authors: Y S Chung, B Nakata, H Tanaka, T Kubo, K Satake, M Sowa, K Umeyama
• Journal: Nihon Geka Gakkai zasshi (Nihon Geka Gakkai Zasshi) Vol. 91 Issue 2 Pg. 228-33 (Feb 1990) ISSN: 0301-4894 [Print] Japan
• PMID: 2325607 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antigens, Neoplasm
• Biomarkers, Tumor
• Reagent Kits, Diagnostic
• pancreatic associated antigen, SPan-1

Topics

• Antigens, Neoplasm (analysis)
• Biomarkers, Tumor (blood)
• Humans
• Pancreatic Neoplasms (diagnosis)
• Pancreatitis (diagnosis)
• Radioimmunoassay
• Reagent Kits, Diagnostic
• Stomach Neoplasms (diagnosis)