Polydatin--a new mitochondria protector for acute severe hemorrhagic shock treatment.

Abstract

OBJECTIVE:

The aim of the study was find out whether neuronal mitochondrial injury does take place in severe shock and to explore effective therapy for severe shock.

RESEARCH DESIGN AND METHODS:

Rats were divided in the following group: sham, shock + normal saline (NS), shock + cyclosporine A (CsA), shock + resveratrol (Res) and shock + polydatin (PD). Rats were subjected to shock for 2 h, followed by administration of NS, CsA, Res and PD, and infusion of shed blood. Morphology, metabolism and function of mitochondria were measured.

RESULTS:

Increased lipid peroxides (LPO) levels, lysosomal injury and mitochondrial permeability transition pore opening took place in neurons, resulting in swollen mitochondria with poorly defined cristae, decreased mitochondrial membrane potential (ΔΨ) and reduced ATP content in shock + NS group, indicating mitochondrial dysfunction. Mitochondrial protectors, such as CsA, Res and PD, partially inhibited these alterations, especially following PD protection, ATP level increased from 44.14 ± 13.81% in shock + NS group to 89.57 ± 9.21% and the survival time was prolonged from 6.3 ± 5.9 h in the shock + NS group to 31.6 ± 13.7 h in shock + PD group.

CONCLUSIONS:

The study shows that neuronal mitochondrial injury is involved in the genesis of severe shock and PD may be the best choice for protection of neuron against mitochondrial injury in severe shock.

• Authors: Xingmin Wang, Rui Song, Yunyan Chen, Ming Zhao, Ke-seng Zhao
• Journal: Expert opinion on investigational drugs (Expert Opin Investig Drugs) Vol. 22 Issue 2 Pg. 169-79 (Feb 2013) ISSN: 1744-7658 [Electronic] England
• PMID: 23241098 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drugs, Chinese Herbal
• Glucosides
• Lipid Peroxides
• Mitochondrial Membrane Transport Proteins
• Mitochondrial Permeability Transition Pore
• Protective Agents
• Stilbenes
• polydatin

Topics

• Acute Disease
• Animals
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal
• Glucosides (administration & dosage, chemistry, therapeutic use)
• Lipid Peroxidation (drug effects)
• Lipid Peroxides (metabolism)
• Membrane Potential, Mitochondrial (drug effects)
• Mitochondria (drug effects, metabolism, ultrastructure)
• Mitochondrial Membrane Transport Proteins (metabolism)
• Mitochondrial Permeability Transition Pore
• Neurons (drug effects, metabolism, ultrastructure)
• Parietal Lobe (drug effects, metabolism, pathology)
• Protective Agents (administration & dosage, chemistry, therapeutic use)
• Rats
• Rats, Wistar
• Severity of Illness Index
• Shock, Hemorrhagic (drug therapy, metabolism, pathology)
• Stilbenes (administration & dosage, chemistry, therapeutic use)
• Survival Analysis