Abstract |
There is increasing evidence for considerable interlinking between the responses to heat stress (HS) and light signaling. In the present work, we provide molecular evidence that BBX18, a negative regulator in photomorphogenesis belonging to the B-box zinc finger protein family in Arabidopsis thaliana, is involved in the regulation of thermotolerance. Using quantitative RT-PCR, GUS staining and immunoblot analysis, our results indicate that the expression of BBX18 was induced by HS. BBX18-RNAi and 35S::BBX18 transgenic Arabidopsis plants were obtained for functional analysis of BBX18. Under-expression of BBX18 displayed increased both basal and acquired thermotolerance in the transgenic plants, while over-expression of BBX18 reduced tolerance to HS in transgenic lines. Moreover, when wild-type, BBX18-RNAi and 35S::BBX18 transgenic plants were treated with HS, HR-related digalactosyldiacylglycerol synthase 1 (DGD1) was down-regulated by BBX18 in both normal and heat shock conditions. Besides, the expression levels of Hsp70, Hsp101 and APX2 were increased in BBX18-RNAi transgenic plants, but lower in 35S::BBX18 transgenic plants. However, the expression of HsfA2 was lower in BBX18-RNAi transgenic plants and higher in the 35S::BBX18 after high-temperature treatment. These results suggesting that, by modulated expression of a set of HS-responsive genes, BBX18 weakened tolerance to HS in Arabidopsis. So our data indicate that BBX18 plays a negative role in thermotolerance.
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Authors | Qiming Wang, Xiaoju Tu, Jihong Zhang, Xinbo Chen, Liqun Rao |
Journal | Molecular biology reports
(Mol Biol Rep)
Vol. 40
Issue 3
Pg. 2679-88
(Mar 2013)
ISSN: 1573-4978 [Electronic] Netherlands |
PMID | 23238922
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adaptation, Physiological
(genetics)
- Arabidopsis
(genetics, metabolism)
- Gene Expression Regulation, Plant
- Genetic Association Studies
- Genotype
- Hot Temperature
- Phenotype
- Stress, Physiological
(genetics)
- Transcription Factors
(genetics)
- Zinc Fingers
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