[Screening of two straw-cellulose degrading actinomycetes].

Abstract

OBJECTIVE:

The aim of this study was to screen microorganisms that could degrade rice straw.

METHODS:

We used selective medium to screen strains and determined straw fracture tension strength, weight loss, lignocellulose decomposition rate and extracellular enzyme activity as re-screening methods after 10 days shake flask culture.

RESULTS:

We isolated two antinomycetes (A3 and A6), the highest cellulose enzyme activity of holoenzyme, beta3-Glucosidase, endonuclease and exonclease for A3 were 12.84, 6.23, 24.56 and 14.00 U/mL, and for A6 12.85, 6.53, 17.80 and 18.80 U/mL. The hemicelluloses enzyme activity was 83.05 for A3 and 52.98 U/mL for A6. Both strains belonged to Streptomyces. With 10 days' treatment, inoculated straws showed a decrease of straw fracture tension strength by 62.67% (A3) and 66.67% (A6), while weight loss of straw was 31.50% (A3) and 35.83% (A6). A3's decomposition rate of cellulose, hemicellulose and lignin was 38.73% , 33.16% and 20.68% , and 47.69% , 28.64% and 22.59% for A6.

CONCLUSION:

Antinomycetes A3 and A6 could degrad cellulose, hemicellulose and lignin.

• Authors: Wenjie Gu, Fabao Zhang, Peizhi Xu, Kaizhi Xie, Shuanhu Tang
• Journal: Wei sheng wu xue bao = Acta microbiologica Sinica (Wei Sheng Wu Xue Bao) Vol. 52 Issue 9 Pg. 1085-93 (Sep 04 2012) ISSN: 0001-6209 [Print] China
• PMID: 23236842 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Proteins
• Cellulose
• Cellulase

Topics

• Actinobacteria (enzymology, genetics, isolation & purification, metabolism)
• Bacterial Proteins (genetics, metabolism)
• Biodegradation, Environmental
• Cellulase (genetics, metabolism)
• Cellulose (metabolism)
• Molecular Sequence Data
• Oryza (metabolism, microbiology)
• Phylogeny
• Plant Stems (metabolism, microbiology)
• Soil Microbiology