Determination of orbifloxacin in sheep plasma by high performance liquid chromatography with ultraviolet detection after intravenous and intramuscular administration.

Abstract

INTRODUCTION:

Single-dose pharmacokinetics of orbifloxacin (2.5mg/kg body weight) were determined in clinically normal female Patanwadi sheep (n=6) following intravenous and intramuscular administration.

METHODS:

Orbifloxacin concentrations were determined by high performance liquid chromatography with ultraviolet detection. The concentration-time data were analyzed by non-compartmental kinetic method.

RESULTS AND DISCUSSION:

Following a single intravenous injection, an elimination half-life (t(1/2β)) of 8.31±0.102h. Steady-state volume of distribution (Vd(ss)) and total body clearance (Cl(B)) were 3.09±0.282L/kg and 0.158±0.006L/kg/h, respectively. Following intramuscular administration, an elimination rate constant (β), the area under the curve from zero to infinity (AUC(0-∞)) and the mean absorption time (MAT) were 0.015±0.001h(-1), 23.49±1.722μg·h/mL and 7.50±0.58h, respectively. The peak plasma concentration (C(max)) of 1.81±0.005µg/mL was achieved at 1.00±0.00h. The mean residence time (MRT) was 26.25±1.083h and the absolute bioavailability was 150.8±12.35%, respectively. Orbifloxacin could be useful for the treatment of bacterial infections in sheep that are sensitive to this drug.

• Authors: G B Dudhatra, Avinash Kumar, M M Awale, D R Kamani, B N Chauhan, H B Patel, S K Mody
• Journal: Journal of pharmacological and toxicological methods (J Pharmacol Toxicol Methods) 2013 Jan-Feb Vol. 67 Issue 1 Pg. 50-3 ISSN: 1873-488X [Electronic] United States
• PMID: 23231925 (Publication Type: Journal Article)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• Ciprofloxacin
• orbifloxacin

Topics

• Administration, Intravenous
• Animals
• Chromatography, High Pressure Liquid (methods)
• Ciprofloxacin (administration & dosage, analogs & derivatives, analysis, blood)
• Female
• Injections, Intramuscular
• Sheep (blood)
• Species Specificity
• Spectrophotometry, Ultraviolet (methods)