Biotransformation of mafosfamide in P388 mice leukemia cells: intracellular 31P-NMR studies.

Abstract

The intracellular transformation of cis-mafosfamide has been studied in P388 mice leukemia cells using 31P-NMR spectroscopy. For this purpose the cells were entrapped in low-gelling-temperature agarose threads. Internal pH of the cells, determined from the position of the intracellular inorganic phosphate, was 7.2. The cell membrane was permeable to 4-hydroxycyclophosphamide and aldophosphamide and less permeable to phosphoramide mustard. 4-Ketocyclophosphamide and carboxyphosphamide signals were not detectable in cells either sensitive or resistant to oxazaphosphorine treatment.

• Authors: H M Sonawat, D Leibfritz, J Engel, P Hilgard
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1052 Issue 1 Pg. 36-41 (Apr 09 1990) ISSN: 0006-3002 [Print] Netherlands
• PMID: 2322593 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Phosphorus
• mafosfamide
• Cyclophosphamide

Topics

• Animals
• Antineoplastic Agents (metabolism)
• Biotransformation
• Cyclophosphamide (analogs & derivatives, metabolism)
• Leukemia P388 (metabolism)
• Leukemia, Experimental (metabolism)
• Magnetic Resonance Spectroscopy (methods)
• Mice
• Phosphorus
• Tumor Cells, Cultured (metabolism)