HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Mitogen-activated protein kinase phosphatase 2 regulates histone H3 phosphorylation via interaction with vaccinia-related kinase 1.

Abstract
Mitogen-activated protein kinase phosphatase 2 (MKP2) is a member of the dual-specificity MKPs that regulate MAP kinase signaling. However, MKP2 functions are still largely unknown. In this study, we showed that MKP2 could regulate histone H3 phosphorylation under oxidative stress conditions. We found that MKP2 inhibited histone H3 phosphorylation by suppressing vaccinia-related kinase 1 (VRK1) activity. Moreover, this regulation was dependent on the selective interaction with VRK1, regardless of its phosphatase activity. The interaction between MKP2 and VRK1 mainly occurred in the chromatin, where histones are abundant. We also observed that the protein level of MKP2 and its interaction with histone H3 increased from G1 to M phase during the cell cycle, which is similar to the VRK1 profile. Furthermore, MKP2 specifically regulated the VRK1-mediated histone H3 phosphorylation at M phase. Taken together, these data suggest a novel function of MKP2 as a negative regulator of VRK1-mediated histone H3 phosphorylation.
AuthorsMin-Woo Jeong, Tae-Hong Kang, Wanil Kim, Yoon Ha Choi, Kyong-Tai Kim
JournalMolecular biology of the cell (Mol Biol Cell) Vol. 24 Issue 3 Pg. 373-84 (Feb 2013) ISSN: 1939-4586 [Electronic] United States
PMID23223570 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Chromatin
  • Histones
  • Intracellular Signaling Peptides and Proteins
  • Hydrogen Peroxide
  • Protein-Serine-Threonine Kinases
  • VRK1 protein, human
  • Mitogen-Activated Protein Kinase Phosphatases
  • DUSP4 protein, human
  • Dual-Specificity Phosphatases
Topics
  • Cell Division
  • Chromatin (enzymology)
  • Dual-Specificity Phosphatases (metabolism)
  • HeLa Cells
  • Histones (metabolism)
  • Humans
  • Hydrogen Peroxide (metabolism)
  • Intracellular Signaling Peptides and Proteins (metabolism)
  • Mitogen-Activated Protein Kinase Phosphatases (metabolism)
  • Oxidative Stress
  • Phosphorylation
  • Protein Processing, Post-Translational
  • Protein Transport
  • Protein-Serine-Threonine Kinases (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: