3,5,4'-Tri-O-acetylresveratrol attenuates seawater aspiration-induced lung injury by inhibiting activation of nuclear factor-kappa B and hypoxia-inducible factor-1α.

Abstract

Resveratrol is a phytoalexin synthesized by a wide variety of plants, which has been proven to be effective in suppressing oxidative stress and inflammation. The aim of the present study was to investigate the effect of Resveratrol's prodrug: 3,5,4'-tri-O-acetylresveratrol, on seawater drowning-induced acute lung injury (SWD-ALI). Histological changes were assessed to study lung injuries; cytokines in lung samples were monitored by ELISA to reflect inflammation; T-SOD and MDA activity were detected to examine oxidative stress in lung tissues. Besides, we also tested the expression of NF-κB and HIF-1α to probe the possible protecting mechanism of 3,5,4'-tri-O-acetylresveratrol on AWD-ALI. The results showed that pretreatment with different doses of 3,5,4'-tri-O-acetylresveratrol improved seawater-induced lung histopathologic changes, alleviated lung edema, reduced the production of inflammatory mediators including TNF-α and IL-1β, inhibited MDA activity, and enhanced T-SOD activity, which was possibly associated with inhibition of NF-κB and HIF-1α. In conclusion, the current study demonstrated that 3,5,4'-tri-O-acetylresveratrol exhibited a protective effect on SWD-ALI by inhibiting of the inflammatory response, which may also involve the suppression of oxidative stress in lung tissues.

• Authors: Lijie Ma, Yilin Zhao, Beixi Li, Qingwei Wang, Xueying Liu, Xiangjun Chen, Yandong Nan, Li Liang, Ruijing Chang, Lifeng Liang, Peipei Li, Faguang Jin
• Journal: Respiratory physiology & neurobiology (Respir Physiol Neurobiol) Vol. 185 Issue 3 Pg. 608-14 (Feb 01 2013) ISSN: 1878-1519 [Electronic] Netherlands
• PMID: 23219988 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Antioxidants
• Hif1a protein, rat
• Hypoxia-Inducible Factor 1, alpha Subunit
• NF-kappa B
• Prodrugs
• Stilbenes
• Resveratrol

Topics

• Animals
• Antioxidants (pharmacology)
• Blotting, Western
• Drowning (metabolism)
• Enzyme-Linked Immunosorbent Assay
• Fluorescent Antibody Technique
• Hypoxia-Inducible Factor 1, alpha Subunit (metabolism)
• Lung (drug effects, metabolism)
• Lung Injury (metabolism)
• Male
• NF-kappa B (metabolism)
• Oxidative Stress (drug effects)
• Prodrugs (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Resveratrol
• Seawater (adverse effects)
• Stilbenes (pharmacology)