Abstract | PURPOSE: We aimed to investigate the effects of caffeic acid phenethyl ester (CAPE) on intestinal mucosal injury induced by superior mesenteric occlusion. METHODS: This experimental study was conducted on 48 male Wistar-albino rats. The animals were randomly allocated into four groups: (i) Sham-operated group, laparotomy without intestinal ischemia/reperfusion (IR) injury (n = 12); (ii) Sham + CAPE group, identical to group 1 except for CAPE treatment (10 μmol/kg, intravenously) (n = 12); (iii) Intestinal IR group, 60 min of superior mesenteric ischemia followed by 3 hr of reperfusion (n = 12); and (iv) (IR + CAPE)-treated group, 10 μmol/kg injection of CAPE intravenously 30 min before the reperfusion period (n = 12). We evaluated the degree of intestinal mucosal injury on a grading scale, histopathologically, and by measuring oxidative stress markers and antioxidant parameters, biochemically. Intestinal edema was estimated by using wet/dry weight ratios. The plasma proinflammatory cytokine levels were measured. Animal survival was observed up to one week. RESULTS: Intestinal mucosal injury scores were significantly decreased with CAPE administration (p < .05). CAPE treatment significantly reduced oxidative stress markers in the intestinal tissues (p < .05) and the plasma proinflammatory cytokine levels (p < .05), and significantly increased antioxidant parameters in the intestinal tissues (p < .05). Intestinal edema was significantly alleviated by CAPE treatment (p < .05). The survival rates of CAPE-treated IR animals were significantly higher than IR-subjected rats (p < .05). CONCLUSION: This study clearly showed that CAPE treatment significantly alleviated the intestinal mucosal injury caused by superior mesenteric ischemia/reperfusion. Further clinical studies are required to clarify whether CAPE has a useful role in reperfusion injury during particular surgeries in which IR-induced organ injury occurs.
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Authors | Zafer Teke, Erdal Birol Bostanci, Cigdem Yenisey, Mustafa Sacar, Nilufer Genc Simsek, Musa Akoglu |
Journal | Journal of investigative surgery : the official journal of the Academy of Surgical Research
(J Invest Surg)
Vol. 25
Issue 6
Pg. 354-65
(Dec 2012)
ISSN: 1521-0553 [Electronic] United States |
PMID | 23215792
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Caffeic Acids
- Cytokines
- Nitrates
- Plant Extracts
- Malondialdehyde
- Peroxidase
- Glutathione Peroxidase
- Xanthine Oxidase
- caffeic acid phenethyl ester
- Phenylethyl Alcohol
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Topics |
- Animals
- Antioxidants
(pharmacology, therapeutic use)
- Caffeic Acids
(pharmacology, therapeutic use)
- Cytokines
(blood)
- Glutathione Peroxidase
(metabolism)
- Intestine, Small
(blood supply, drug effects, metabolism)
- Male
- Malondialdehyde
(metabolism)
- Models, Animal
- Nitrates
(metabolism)
- Oxidative Stress
(drug effects)
- Peroxidase
(metabolism)
- Phenylethyl Alcohol
(analogs & derivatives, pharmacology, therapeutic use)
- Plant Bark
- Plant Extracts
(pharmacology, therapeutic use)
- Rats
- Rats, Wistar
- Regional Blood Flow
(drug effects)
- Reperfusion Injury
(metabolism, prevention & control)
- Xanthine Oxidase
(metabolism)
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