Calpain I activity and its relationship with hippocampal neuronal death in pilocarpine-induced status epilepticus rat model.

Abstract

This study aims to establish pilocarpine-induced rat model of status epilepticus (SE), observe the activity of calpain I in the rat hippocampus and the subsequent neuronal death, and explore the relationship between calpain I activity and neuronal death in the hippocampus. Fifty-eight adult male Wistar rats were assigned randomly into either control group (n = 8) or epilepsy group (n = 50). SE was induced in the epilepsy group using pilocarpine. Before the injection, the rats were given atropine sulfate to reduce the side effect of pilocarpine. All rats in the seizure group were grouped into either SE or non-SE, depending on whether they developed convulsive seizures. The rats in SE group were treated with chloral hydrate to stop seizures after 60 min. Control animals were treated with the same dose of 0.9 % saline. All rats were monitored for seizures. At 24 h after SE, the rats' left brain tissues were stained by HE and TUNEL. Neuronal necrosis and apoptosis in the hippocampal CA3 area were observed. Calpain I activity in the right hippocampus was also observed using western blotting. Eighty percent of the rats in the seizure group developed SE, of which 35 % died. No rat died in both the control and non-SE groups. At 24 h after SE, the number of HE-stained neurons decreased (SE group: 55.19 ± 8.23; control group: 102.13 ± 3.73; non-SE group: 101.2 ± 2.86) and the number of TUNEL-positive neurons increased (SE group: 4.91 ± 1.35; non-SE and control group: 0). No obvious changes were observed in the neurons of the control and non-SE group animals. The 76 kDa cleavage of calpain I (the average optical density ratio is 0.096 ± 0.015) emerged in the SE group. Neuronal death has a direct relationship with calpain I activity. There is high success rate and lower death rate for pilocarpine to induce SE. At 24 h after SE, activity of calpain I, neuronal necrosis and apoptosis increased in the hippocampus. Neuronal death has a direct relationship with calpain I activity, which suggests that calpain I plays an important role in neuronal damage during SE.

• Authors: Hua Gao, Zhi Geng
• Journal: Cell biochemistry and biophysics (Cell Biochem Biophys) Vol. 66 Issue 2 Pg. 371-7 (Jun 2013) ISSN: 1559-0283 [Electronic] United States
• PMID: 23212179 (Publication Type: Journal Article)

Chemical References

• Hypnotics and Sedatives
• Parasympatholytics
• Pilocarpine
• Chloral Hydrate
• Atropine
• Calpain

Topics

• Animals
• Apoptosis (drug effects)
• Atropine (pharmacology)
• Calpain (metabolism)
• Chloral Hydrate (pharmacology)
• Disease Models, Animal
• Hippocampus (drug effects, enzymology, pathology)
• Hypnotics and Sedatives (pharmacology)
• Linear Models
• Male
• Neurons (drug effects, enzymology, pathology)
• Parasympatholytics (pharmacology)
• Pilocarpine (toxicity)
• Rats
• Rats, Wistar
• Status Epilepticus (chemically induced, enzymology, pathology)