Effects of the herbal medicine composition "Saiko-ka-ryukotsu-borei-To" on the function of endothelial progenitor cells in hypertensive rats.

Abstract

Endothelial progenitor cells (EPCs) are known to repair vascular injuries. Recent studies suggest that Saiko-ka-ryukotsu-borei-To (SKRBT), a traditional herbal medicine that has been used to treat stress-related neuropsychiatric disorders, has protective effects on cardiovascular diseases such as hypertension and arteriosclerosis. Spontaneously hypertensive rats (SHRs) were fed diets containing lyophilized SKRBT extract for 6 weeks. Peripheral blood mononuclear cells (MNCs) were isolated and cultured to assay EPC colony formation. Oxidative stress in MNCs was evaluated by thiobarbituric acid reactive substance (TBARS) assay and flowcytometric analyses. Treatment with SKRBT increased EPC colony numbers significantly (p<0.05) with decrease in oxidative stress and without affecting blood pressure in SHRs. Treatment with SKRBT did not reduce the expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunits in cardiovascular organs. Serum IL-6 level was significantly reduced. SKRBT is a feasible herbal medicine that protects against cardiovascular diseases through an increase in EPC function along with anti-oxidative effects, and may affect the link between chronic inflammation and cardiovascular disease.

• Authors: Hiroshi Iijima, Akihiro Daikonya, Satoshi Takamatsu, Ai Kanno, Kayoko Magariyama, Kazuki Yoshikawa, Tomoko Takamiya, Yukiko Ueda, Shuji Yakubo, Taro Matsumoto, Takahiro Ueno, Yukio Yamori, Noboru Fukuda, Susumu Kitanaka
• Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (Phytomedicine) Vol. 20 Issue 3-4 Pg. 196-201 (Feb 15 2013) ISSN: 1618-095X [Electronic] Germany
• PMID: 23207252 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier GmbH. All rights reserved.

Chemical References

• Cytokines
• Drugs, Chinese Herbal
• RNA, Messenger
• Reactive Oxygen Species
• Thiobarbituric Acid Reactive Substances
• saiko-ka-ryukotsu-borei-to
• NADPH Oxidases

Topics

• Animals
• Bone Marrow Cells (drug effects)
• Cytokines (blood)
• Drug Evaluation, Preclinical
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Endothelial Cells (drug effects)
• Hypertension (prevention & control)
• Leukocytes, Mononuclear (metabolism)
• Male
• NADPH Oxidases (metabolism)
• Phytotherapy
• RNA, Messenger (metabolism)
• Rats
• Rats, Inbred SHR
• Reactive Oxygen Species (metabolism)
• Thiobarbituric Acid Reactive Substances (metabolism)