Abstract | OBJECTIVES: MATERIALS AND METHODS: Gd-P, control peptide KAREC conjugated nanoglobular contrast agent (Gd-CP), and control nontargeting nanoglobular contrast agent (Gd-C) were synthesized. Male adult C57BL/6N mice (22-25 g; N = 54) were prepared and were divided into fibrosis (n = 36) and normal (n = 18) groups. Liver fibrosis was induced in the fibrosis group through subcutaneous injection of 1:3 mixture of carbon tetrachloride (CCl(4)) in olive oil at a dose of 4 μL/g of body weight twice a week for 8 weeks. Dynamic contrast-enhanced MRI was performed in all animals. Dynamic contrast-enhanced magnetic resonance imaging was analyzed to yield postinjection ΔR(1)(t) maps for quantitative measurements. Histological analysis was also performed. RESULTS: Differential enhancements were observed and characterized between the normal and fibrotic livers using Gd-P at 0.03 mmol/kg, when compared with nontargeted controls (Gd-CP and Gd-C). For Gd-P injection, both the peak and steady-state ΔR(1) of the normal livers were significantly lower than those after 4 and 8 weeks of CCl(4) dosing. Liver fibrogenesis with increased amount of fibronectin in the extracellular space in insulted livers were confirmed by histological observations. CONCLUSIONS:
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Authors | April M Chow, Mingqian Tan, Darwin S Gao, Shu Juan Fan, Jerry S Cheung, Kwan Man, Zheng-Rong Lu, Ed X Wu |
Journal | Investigative radiology
(Invest Radiol)
Vol. 48
Issue 1
Pg. 46-54
(Jan 2013)
ISSN: 1536-0210 [Electronic] United States |
PMID | 23192162
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Contrast Media
- Fibronectins
- Peptides, Cyclic
- cyclo(cysteinyl-glycyl-leucyl-isoleucyl-isoleucyl-glutaminyl-lysyl-asparaginyl-glutamyl-cysteinyl)
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Topics |
- Animals
- Contrast Media
- Fibronectins
(metabolism)
- Liver Cirrhosis
(diagnosis, metabolism, pathology)
- Magnetic Resonance Imaging
- Male
- Mice
- Mice, Inbred C57BL
- Molecular Diagnostic Techniques
- Peptides, Cyclic
(metabolism)
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