Effects of acetylcholine on an animal mode of inflammation.

Abstract

Acetylcholinesterase (AChE) is found both on the membranes of neuronal and non-neuronal cells. In this study we performed intravenous administrations of velnacrine (VLN) and acetylcholine (ACh), respectively, AChE inhibitor and substrate, in an animal model of lipopolysaccharide (LPS)-induced inflammation in Wistar rats. Using intravital microscopy the number of rolling and adherent leukocytes in post-capillary venules was monitorized and blood samples were collected for TNF-α plasma concentrations determination. Our results showed that in presence of LPS, ACh has an anti-inflammatory effect, seen by a decrease in TNF-α plasma levels and maintains the number of rolling and adherent leukocytes. The presence of VLN before LPS almost blocked the LPS-induced rolling and TNF-α releasing. Thereby VLN seems to have, like ACh, an anti-inflammatory effect by diminishing TNF-α concentrations.

• Authors: A S Silva-Herdade, C Saldanha
• Journal: Clinical hemorheology and microcirculation (Clin Hemorheol Microcirc) Vol. 53 Issue 1-2 Pg. 209-16 ( 2013) ISSN: 1875-8622 [Electronic] Netherlands
• PMID: 23187452 (Publication Type: Journal Article)

Chemical References

• Cholinesterase Inhibitors
• Lipopolysaccharides
• Tumor Necrosis Factor-alpha
• Tacrine
• Acetylcholine
• velnacrine

Topics

• Acetylcholine (pharmacology)
• Animals
• Cell Movement (drug effects)
• Cholinesterase Inhibitors (pharmacology)
• Endothelium, Vascular (drug effects)
• Inflammation (drug therapy)
• Leukocytes (drug effects, physiology)
• Lipopolysaccharides
• Male
• Microscopy
• Rats
• Rats, Wistar
• Tacrine (analogs & derivatives, pharmacology)
• Tumor Necrosis Factor-alpha (blood)