Abstract | AIM OF THE STUDY: MATERIALS AND METHODS: Forty male Wistar rats were randomly divided into five groups, including model group, normal control group, dexamethasone group, HLJDT group and the mixture of 13 components of HLJDT group after immunized with bovine type II collagen. Urine samples for metabolomic study were collected on 8, 15, 22 day during the animal experiment. RESULTS: The pharmacological changes (swelling paws and arthritis scores) showed that prophylactic treatment with HLJDT and its components significantly suppressed the swelling of rats' paws. By combining with partial least squares discriminant analysis, 24 potential biomarkers were identified and primarily involved in 12 metabolism pathways, such as tricarboxylic acids cycle metabolism, lipid metabolism, tryptophan metabolism and phenylalanine metabolism, which revealed a new insight into the RA network in vivo. These potential metabolites identified in CIA model need to be further investigated to prove their diagnostic and/or prognostic values for RA. Taking potential biomarkers found in the study as screening indexes, it revealed that HLJDT and its components could reverse the pathological process of RA through partly regulating the disturbed metabolic pathways. CONCLUSIONS: This study indicated that the metabolomic strategy based on RRLC-TOF-MS is a useful tool to search potential biomarkers related to RA and to dissect the underlying mechanisms of TCM in treating RA.
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Authors | Rongcai Yue, Ling Zhao, Yaohua Hu, Peng Jiang, Shuping Wang, Li Xiang, Wencong Liu, Weidong Zhang, Runhui Liu |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 145
Issue 2
Pg. 465-75
(Jan 30 2013)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 23183090
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Biomarkers
- Drugs, Chinese Herbal
- huang-lien-chieh-tu-tang
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Topics |
- Animals
- Anti-Inflammatory Agents
(pharmacology, therapeutic use)
- Arthritis, Experimental
(drug therapy, urine)
- Biomarkers
(urine)
- Chromatography, Liquid
(methods)
- Drugs, Chinese Herbal
(pharmacology, therapeutic use)
- Male
- Mass Spectrometry
(methods)
- Metabolomics
- Phytotherapy
- Rats
- Rats, Wistar
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