Effect of isoflavone on balloon catheter-induced neointimal hyperplasia in ovariectomised rabbit carotid artery.

Abstract

BACKGROUND:

This study was designed to investigate the effects of phytoestrogen isoflavone on balloon catheter-induced hyperplasia of carotid artery.

METHODS:

Forty-eight female New Zealand rabbits were randomly divided into four groups: control (balloon-induced carotid artery injury only); ovariectomy control (ovariectomy and carotid artery injury), oestrogen (ovariectomy, carotid artery injury and nilestriol, 5mg/kg daily for 28 days), and isoflavone (ovariectomy, carotid artery injury and isoflavone 120 mg/kg daily for 28 days). The arterial wall thickness was assessed by coloured ultrasonography, and the oestrogen-α and oestrogen-β receptors in the abdominal aorta were measured by Western blotting.

RESULTS:

The medial layer thickness in the isoflavone group was less than in the ovariectomy control group (0.28±0.03 vs. 0.35±0.04 mm, p<0.01), and the intimal/medial layer (I/M) ratio is the isoflavone group was also less than in the ovariectomy control group (16.85±3.79 vs. 48.94±8.92, p<0.01). There was no statistically significant difference in the medial layer thickness or I/M ratio between the isoflavone and the oestrogen groups. The optical density of the oestrogen-α receptors in the isoflavone group (0.317±0.002) was less than in the oestrogen (0.633±0.002) or ovariectomy control group (0.590±0.001, p<0.01). The optical density of the oestrogen-β receptors in the isoflavone group (1.350±0.002) and the ovariectomy control group (1.2033±0.002) was less than in the oestrogen group (1.7699±0.003, p<0.01).

CONCLUSIONS:

Isoflavone therapy in the ovariectomised rabbit model attenuated balloon catheter-induced intimal and medial layer hyperplasia in the carotid arteries. Down-regulation of the oestrogen-α receptors may be involved in the hyperplasia-preventative effect.

• Authors: Gai-Ying Zhang, Ren-Feng Qiu, Ying-Chun Sun, Le-Xin Wang
• Journal: Heart, lung & circulation (Heart Lung Circ) Vol. 22 Issue 2 Pg. 141-5 (Feb 2013) ISSN: 1444-2892 [Electronic] Australia
• PMID: 23177646 (Publication Type: Journal Article)
• Copyright: Copyright © 2012. Published by Elsevier B.V.

Chemical References

• Drosophila Proteins
• Estrogen Receptor alpha
• Estrogen Receptor beta
• Eye Proteins
• Isoflavones
• Nerve Tissue Proteins
• Phytoestrogens
• aos protein, Drosophila

Topics

• Animals
• Carotid Arteries (diagnostic imaging, pathology)
• Catheterization (adverse effects)
• Drosophila Proteins (chemistry)
• Estrogen Receptor alpha (analysis)
• Estrogen Receptor beta (analysis)
• Eye Proteins (chemistry)
• Female
• Hyperplasia (diagnostic imaging, drug therapy, etiology)
• Isoflavones (therapeutic use)
• Nerve Tissue Proteins (chemistry)
• Ovariectomy
• Phytoestrogens (therapeutic use)
• Rabbits
• Soybeans
• Tunica Intima (diagnostic imaging, pathology)
• Ultrasonography, Doppler, Color