Cell cycle analysis of the CD133(+) and CD133(-) cells isolated from human colorectal cancer.

Abstract

AIM:

The CD133 antigen has been identified as a putative stem cell marker in colorectal cancer tissues. The aim of this study was to investigate the cell cycle state of CD133(+) and CD133(-) cells, isolated from primary human colorectal tumors.

MATERIALS AND METHODS:

After mechanical and enzymatic dissociation of the tumor samples, CD133(+) and CD133(-) subsets were identified and separated by magnetic cell sorting. Flow cytometric analysis was performed to compare the cell cycle of both CD133(+) and CD133(-) cells isolated from primary and liver metastatic cancer cells.

RESULTS:

The results indicated that CD133(+) cells isolated from both primary and liver metastatic colorectal cancers were found in higher percentage in the G0/G1 phases. However, the CD133(-) cells isolated from primary colorectal cancers were predominantly found in the S and G2/M phases. Surprisingly, the CD133(-) cells isolated from liver metastatic colorectal cancers were mostly found in the G0/G1 phase.

CONCLUSION:

The present study provides evidence that CD133(+) cells are in a quiescent state in colorectal cancer, representing a mechanism that would at least partially explain chemotherapy resistance and tumor recurrence in post-therapy patients.

• Authors: Marjan Gharagozloo, Hamid R Mirzaei, Bahram Bagherpour, Abbas Rezaei, Hamid Kalantari, Mohammad H Sanei, Mohsen Hosseini, Gholamreza Mohajeri, Abbas Tabatabai, Mozaffar Hashemi
• Journal: Journal of cancer research and therapeutics (J Cancer Res Ther) 2012 Jul-Sep Vol. 8 Issue 3 Pg. 399-403 ISSN: 1998-4138 [Electronic] India
• PMID: 23174722 (Publication Type: Journal Article)

Chemical References

• AC133 Antigen
• Antigens, CD
• Biomarkers, Tumor
• Glycoproteins
• PROM1 protein, human
• Peptides

Topics

• AC133 Antigen
• Aged
• Antigens, CD (metabolism)
• Biomarkers, Tumor (metabolism)
• Cell Cycle
• Colorectal Neoplasms (drug therapy, pathology)
• Drug Resistance, Neoplasm
• Female
• Glycoproteins (metabolism)
• Humans
• Liver Neoplasms (drug therapy, pathology, secondary)
• Male
• Middle Aged
• Neoplastic Stem Cells
• Peptides (metabolism)