Chitosan oligosaccharide-arachidic acid-based nanoparticles for anti-cancer drug delivery.

Abstract

Chitosan oligosaccharide-arachidic acid (CSOAA) conjugate was successfully synthesized and used for the development of self-assembled nanoparticles for doxorubicin (DOX) delivery. The molar substitution of AA on CSO and critical micelle concentration (CMC) of CSOAA were measured. Physicochemical properties of DOX-loaded CSOAA-based nanoparticles, such as particle size, zeta potential and morphology, were also characterized. The DOX-loaded CSOAA-based nanoparticles showed spherical shape with a mean diameter of 130 nm and positive charge. According to the result of in vitro release test, DOX-loaded CSOAA-based nanoparticles exhibited sustained and pH-dependent drug release profiles. The CSOAA showed negligible cytotoxicity in FaDu, human head and neck cancer, cells. Cellular uptake of DOX in FaDu cells was higher in the nanoparticle-treated group compared to the free DOX group. The anti-tumor efficacy of DOX-loaded nanoparticles was also verified in FaDu tumor xenografted mouse model. These results suggested that synthesized amphiphilic CSOAA might be used for the preparation of self-assembled nanoparticles for anti-cancer drug delivery.

• Authors: Ubonvan Termsarasab, Hyun-Jong Cho, Dong Hwan Kim, Saeho Chong, Suk-Jae Chung, Chang-Koo Shim, Hyun Tae Moon, Dae-Duk Kim
• Journal: International journal of pharmaceutics (Int J Pharm) Vol. 441 Issue 1-2 Pg. 373-80 (Jan 30 2013) ISSN: 1873-3476 [Electronic] Netherlands
• PMID: 23174411 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Antibiotics, Antineoplastic
• Delayed-Action Preparations
• Drug Carriers
• Eicosanoic Acids
• Oligosaccharides
• Doxorubicin
• Chitosan
• arachidic acid

Topics

• Animals
• Antibiotics, Antineoplastic (administration & dosage, pharmacology)
• Cell Line, Tumor
• Chitosan (chemistry)
• Delayed-Action Preparations
• Doxorubicin (administration & dosage, pharmacology)
• Drug Carriers (chemistry)
• Drug Delivery Systems
• Eicosanoic Acids (chemistry)
• Female
• Head and Neck Neoplasms (drug therapy, pathology)
• Humans
• Hydrogen-Ion Concentration
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Nanoparticles
• Oligosaccharides (chemistry)
• Particle Size
• Xenograft Model Antitumor Assays