[Comparison of Kinyoun, auramine O, and Ziehl-Neelsen staining for diagnosing tuberculosis at the National Tuberculosis Center in Burkina Faso].

Abstract

CONTEXT:

This study was conducted at the National Tuberculosis Center in Burkina Faso from October 2007 through May 2008.

OBJECTIVE:

Our objective was to compare the diagnostic performance of three staining methods: Kinyoun, auramine O, and Ziehl-Neelsen.

METHODS:

Ziehl-Neelsen staining served as the reference method to assess the diagnostic performance of Kinyoun and auramine O staining. In all, 616 sputum smears from 233 patients were read with each method to detect acid-fast bacilli. SPSS was used for data analysis.

RESULTS:

The results of auramine O staining showed positive diagnoses in 15.9% of the samples; sensitivity was 100%, specificity 95.6%, and the positive and negative predictive values 75.7% and 100% respectively. Kinyoun staining produced a positive diagnosis rate of 12%, sensitivity of 96.4%, specificity of 99.5%, and positive and negative predictive values of 96.4% and 99.5%.

CONCLUSION:

Our study indicates that auramine O staining had a better sensitivity for detecting acid-fast bacilli than Kinyoun staining. Accordingly, the use of auramine O staining should increase the detection rate for pulmonary tuberculosis in Burkina Faso.

• Authors: T L Sawadogo, L G B Savadogo, S Diande, F Ouedraogo, A Mourfou, A Gueye, I Sawadogo, B Nebié, L Sangare, A S Ouattara
• Journal: Medecine et sante tropicales (Med Sante Trop) 2012 Jul-Sep Vol. 22 Issue 3 Pg. 302-6 ISSN: 2261-2211 [Electronic] France
• Vernacular Title: Comparaison des performances des colorations de Kinyoun et à l'auramine O, avec celle de Ziehl-Neelsen pour le diagnostic de la tuberculose au Centre national de lutte antituberculeuse du Burkina Faso.
• PMID: 23174139 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Coloring Agents
• Benzophenoneidum

Topics

• Benzophenoneidum
• Burkina Faso
• Coloring Agents
• Humans
• Sputum (microbiology)
• Tuberculosis, Pulmonary (diagnosis)