Follistatin is induced by IL-1β and TNF-α in stromal cells from endometrioma.

Abstract

The aim of this study is to examine the regulation of follistatin, an activin-binding protein, in endometriosis. Endometrioma stromal cells (EoSCs) were obtained from 9 patients undergoing laparoscopy of the ovarian endometrioma. In cultured EoSCs, interleukin 1β (IL-1β) and tumor necrosis factor-α (TNF-α), which could induce activin-A, also induced follistatin messenger RNA (mRNA) and protein. The cystic fluid of endometrioma from 8 patients was obtained to measure the concentration of activin-A and follistatin by enzyme-linked immunosorbent assay (ELISA). Also, activin activity in the fluid was examined by erythroid differentiation assay using mouse erythroleukemia F5-5.fl cells. In the cystic fluid of endometrioma, the mean values of activin-A and follistatin concentration were 36.8 ng/mL and 4.0 ng/mL, respectively. In a bioassay, all 8 samples exhibited activin activity, which was equivalent to recombinant activin-A activity of 12.8 ± 1.4 ng/mL. Although follistatin was present in the cystic fluid of endometrioma, the activity of activin, which is an exacerbation factor of endometriosis, was predominant in vivo.

• Authors: Ikumi Akiyama, Osamu Yoshino, Yutaka Osuga, Gentaro Izumi, Yoko Urata, Yasushi Hirota, Tetsuya Hirata, Miyuki Harada, Kaori Koga, Kenji Ogawa, Shiro Kozuma
• Journal: Reproductive sciences (Thousand Oaks, Calif.) (Reprod Sci) Vol. 20 Issue 6 Pg. 675-9 (Jun 2013) ISSN: 1933-7205 [Electronic] United States
• PMID: 23171678 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• FST protein, human
• Follistatin
• Interleukin-1beta
• RNA, Messenger
• Recombinant Proteins
• Tumor Necrosis Factor-alpha
• activin A
• Activins

Topics

• Activins (metabolism)
• Animals
• Cell Line
• Endometriosis (metabolism)
• Enzyme-Linked Immunosorbent Assay
• Female
• Follistatin (genetics, metabolism)
• Humans
• Interleukin-1beta (metabolism)
• Mice
• RNA, Messenger (metabolism)
• Real-Time Polymerase Chain Reaction
• Recombinant Proteins (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Stromal Cells (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)
• Up-Regulation