Induction of interleukin-1 alpha and beta gene transcription in mouse peritoneal exudate cells after intraperitoneal infection with herpes simplex virus-1.

Abstract

Macrophages have been shown to play a determining role in the immune defense against herpes simplex virus-1 (HIV-1) intraperitoneal infection in the mouse. In the present study, the effect of HSV-1 infection on interleukin-1 alpha and beta gene transcription in peritoneal exudate cells was investigated. HSV-1 infection was found to induce interleukin-1 alpha and beta gene transcription in these cells. Induction of the interleukin-1 beta gene initiated 6 h postinfection (p.i.) and terminated at 48 h p.i. In contrast, interleukin-1 alpha RNA was detectable at high levels at 6h p.i. but not at 24 h p.i. Inactivation of virus prior to infection prevented HSV-1-induced IL-1 gene induction, indicating that only infectious virus is able to stimulate IL-1 gene transcription. These findings are discussed in relation to the role of macrophages in the immunological mechanisms of defense against HSV-1 infection.

• Authors: E Sprecher, Y Becker
• Journal: Archives of virology (Arch Virol) Vol. 110 Issue 3-4 Pg. 259-69 ( 1990) ISSN: 0304-8608 [Print] Austria
• PMID: 2317155 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• DNA Probes
• Interleukin-1
• RNA, Viral

Topics

• Animals
• Blotting, Northern
• DNA Probes
• Densitometry
• Female
• Gene Expression
• Herpes Simplex (immunology)
• Interleukin-1 (biosynthesis, genetics)
• Mice
• Mice, Inbred C57BL
• Peritonitis (etiology, immunology)
• RNA, Viral (biosynthesis)
• Transcription, Genetic