Abstract | BACKGROUND: METHODS: We investigated GnT-IVa expression in GTDs and placentas by immunohistochemistry, western blot, and RT-PCR. We assessed the effects of GnT-IVa knockdown in choriocarcinoma cells in vitro and in vivo. RESULTS: The GnT-IVa was highly expressed in trophoblasts of invasive mole and choriocarcinoma, and moderately in extravillous trophoblasts during the first trimester, but not in hydatidiform mole or other normal trophoblasts. The GnT-IVa knockdown in choriocarcinoma cells significantly reduced migration and invasive capacities, and suppressed cellular adhesion to extracellular matrix proteins. The extent of β1,4-N-acetylglucosamine branching on β1 integrin was greatly reduced by GnT-IVa knockdown, although the expression of β1 integrin was not changed. In vivo studies further demonstrated that GnT-IVa knockdown suppressed tumour engraftment and growth. CONCLUSION:
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Authors | K Niimi, E Yamamoto, S Fujiwara, K Shinjo, T Kotani, T Umezu, H Kajiyama, K Shibata, K Ino, F Kikkawa |
Journal | British journal of cancer
(Br J Cancer)
Vol. 107
Issue 12
Pg. 1969-77
(Dec 04 2012)
ISSN: 1532-1827 [Electronic] England |
PMID | 23169300
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Chorionic Gonadotropin, beta Subunit, Human
- Integrin beta1
- N-Acetylgalactosaminyltransferases
- N-Acetylglucosaminyltransferases
- alpha-1,3-mannosylglycoprotein beta-1,4-N-acetylglucosaminyltransferase
- (N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase
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Topics |
- Adult
- Biomarkers, Tumor
(metabolism)
- Blotting, Western
- Cell Movement
- Cell Proliferation
- Choriocarcinoma
(enzymology, pathology)
- Chorionic Gonadotropin, beta Subunit, Human
(metabolism)
- Female
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Neoplastic
- Gestational Trophoblastic Disease
(enzymology, pathology)
- Humans
- Hydatidiform Mole, Invasive
(enzymology, pathology)
- Immunohistochemistry
- Integrin beta1
(metabolism)
- N-Acetylgalactosaminyltransferases
(metabolism)
- N-Acetylglucosaminyltransferases
(metabolism)
- Neoplasm Invasiveness
- Pregnancy
- Real-Time Polymerase Chain Reaction
- Up-Regulation
- Uterine Neoplasms
(enzymology, pathology)
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