Abstract | OBJECTIVES: DESIGN: Prospective case-control study. SETTING: Tertiary care academic medical center. PATIENTS: A total of 11 control subjects and 85 patients with clinical and audiometric characteristics of IM-SNHL (autoimmune inner ear disease and sudden SNHL combined) treated with corticosteroids were enrolled in the study. Patients were categorized as steroid responders (n = 47) and steroid nonresponders (n = 38). Peripheral venous blood was used to determine the total amount of plasma TNF by enzyme-linked immunosorbent assay. Peripheral blood mononuclear cells (PBMCs) were isolated and treated with in vitro dexamethasone. Treated and untreated PBMCs were then analyzed for release of soluble TNF protein into conditioned supernatants as well as expression of TNF messenger RNA ( mRNA). MAIN OUTCOME MEASURES: Mean plasma levels of TNF, unstimulated and dexamethasone-stimulated PBMC-secreted levels of TNF, and TNF mRNA levels in unstimulated and dexamethasone-stimulated PBMCs. RESULTS:
Steroid nonresponders had the highest mean baseline plasma levels of TNF compared with steroid responders and control subjects (27.6, 24.1, and 14.4 pg/mL, respectively) (P = .03). For patients with IM-SNHL with a high baseline plasma levels of TNF (>14.4 pg/mL), the mean TNF secreted by PBMCs was 59.1 pg/mL, which decreased to 7.2 pg/mL with in vitro dexamethasone stimulation in the responder group, while the mean TNF secreted by PBMCs was 11.2 pg/mL, which slightly increased to 11.7 pg/mL with in vitro dexamethasone stimulation in the nonresponder group (P = .04). CONCLUSIONS: The level of TNF can be used as both a diagnostic and prognostic cytokine for IM-SNHL. For patients presenting with a sudden change in hearing threshold, a high baseline plasma TNF from the peripheral circulation is supportive of the diagnosis if it is greater than 18.8 pg/mL, with a positive predictive value higher than 97%. In addition, this study demonstrates that for patients with IM-SNHL and high plasma levels of TNF, their clinical response to oral glucocorticoids can be predicted by their in vitro PBMC response to dexamethasone. This algorithm may further guide optimal medical treatment and possibly avoid the deleterious adverse effects of administering glucocorticoids to those patients who would not benefit from their effect.
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Authors | Maja Svrakic, Shresh Pathak, Eliot Goldofsky, Ronald Hoffman, Sujana S Chandrasekhar, Neil Sperling, George Alexiades, Matthew Ashbach, Andrea Vambutas |
Journal | Archives of otolaryngology--head & neck surgery
(Arch Otolaryngol Head Neck Surg)
Vol. 138
Issue 11
Pg. 1052-8
(Nov 2012)
ISSN: 1538-361X [Electronic] United States |
PMID | 23165380
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Glucocorticoids
- Tumor Necrosis Factor-alpha
- Dexamethasone
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Topics |
- Academic Medical Centers
- Adolescent
- Adult
- Aged
- Analysis of Variance
- Biomarkers
(blood)
- Case-Control Studies
- Cells, Cultured
(drug effects)
- Dexamethasone
(therapeutic use)
- Enzyme-Linked Immunosorbent Assay
- Female
- Glucocorticoids
(therapeutic use)
- Hearing Loss, Sensorineural
(blood, diagnosis, drug therapy, immunology)
- Humans
- Leukocytes, Mononuclear
(immunology)
- Male
- Middle Aged
- Prognosis
- Prospective Studies
- Real-Time Polymerase Chain Reaction
(methods)
- Severity of Illness Index
- Statistics, Nonparametric
- Treatment Outcome
- Tumor Necrosis Factor-alpha
(blood, metabolism)
- Young Adult
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