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Measurement of reference intervals for urinary free adrenal steroid levels in Japanese newborn infants by using stable isotope dilution gas chromatography/mass spectrometry.

AbstractBACKGROUND:
In newborn infants, there are no reference intervals for urinary free steroids, which are thought to reflect the bioavailable fraction of steroids in the blood. We establish a method for simultaneous measurement of urinary free adrenal steroids such as pregnenolone, progesterone, 16α-hydroxyprogesterone, 17α-hydroxyprogesterone, 21-deoxycortisone, 21-deoxycortisol, dehydroepiandrosterone, androstenedione, and 11β-hydroxyandrostenedione by using stable isotope dilution gas chromatography/mass spectrometry (SID-GC/MS) and determined the reference intervals for urinary levels of free adrenal steroids in Japanese newborn infants.
METHODS:
Newborn pooled urine was used for validation. Spot urine samples were collected from 67 full-term Japanese newborn infants (34 male and 33 female infants) at 3-4 days of age to determine reference intervals. The extracted and purified free steroids were delivered with heptafluorobutyric anhydride and analyzed by SID-GC/MS.
RESULTS:
We validated a SID-GC/MS method with good repeatability and recovery rate. The preliminary reference intervals (median [range], μmol/mol creatinine) were as follows: pregnenolone, 4.2 (0.7-31.6); progesterone, 0.5 (not detected (n.d.)-0.6); 16α-hydroxyprogesterone, 1.4 (n.d.-10.3); 17α-hydroxyprogesterone, 1.1 (n.d.-1.9); 21-deoxycortisone, n.d. (n.d.-n.d.); 21-deoxycortisol, n.d. (n.d.-n.d.); dehydroepiandrosterone, 2.2 (0.6-27.3); androstenedione, 0.7 (n.d.-5.2); and 11β-hydroxyandrostenedione, 2.9 (n.d.-26.7).
CONCLUSIONS:
We established a reliable SID-GC/MS method and were able to determine preliminary reference intervals for 9 urinary free adrenal steroids in newborn infants.
AuthorsYuhei Koyama, Keiko Homma, Masayuki Miwa, Kazushige Ikeda, Mitsuru Murata, Tomonobu Hasegawa
JournalClinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 415 Pg. 302-5 (Jan 16 2013) ISSN: 1873-3492 [Electronic] Netherlands
PMID23159298 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 Elsevier B.V. All rights reserved.
Chemical References
  • Adrenal Cortex Hormones
  • Oxygen Isotopes
Topics
  • Adrenal Cortex Hormones (urine)
  • Adrenal Glands (metabolism)
  • Asian People
  • Female
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Oxygen Isotopes
  • Reference Standards
  • Reference Values
  • Reproducibility of Results
  • Sensitivity and Specificity

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