Abstract | OBJECTIVE: The aim of this study was to determine the expression of essential osteogenic genes related to the canonic WNT pathway, i.e., WDR5, sFRP-2, and their downstream genes, in ameloblastoma and to clarify their biologic impact on this neoplasm. STUDY DESIGN: Forty-six paraffin-embedded ameloblastoma samples and ameloblastic (AM-1) and preosteoblastic (KUSA/A1) cell lines were used. Immunohistochemistry, Western blot, reverse-transcription polymerase chain reaction, and alkaline phosphatase (ALP) activity assay were performed. RESULTS: WDR5, essential for osteoblast differentiation and canonic WNT pathway activation, was negative in most ameloblastoma cases and weakly expressed in AM-1 cells. Conversely, sFRP-2s was overexpressed. RUNX2 and C-MYC, downstream inductions of canonic WNT pathway activation, demonstrated weak mRNA expressions in ameloblastoma, suggesting WNT pathway impairment and WDR5 functional inactivity. Recombinant WDR5 weakly induced ALP activity of KUSA/A1 cells cultured in AM-1 conditioned medium. CONCLUSIONS: These findings suggest that WNT-related bone-forming genes are down-regulated in ameloblastoma. Concurrent sFRP-2 overexpression suggests that both bone-forming and bone-inhibiting genes equally contributed to reduced bone formation in this neoplasm.
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Authors | Gulsan A Sathi, Hidetsugu Tsujigiwa, Satoshi Ito, Chong Huat Siar, Naoki Katase, Ryo Tamamura, Hidemitsu Harada, Hitoshi Nagatsuka |
Journal | Oral surgery, oral medicine, oral pathology and oral radiology
(Oral Surg Oral Med Oral Pathol Oral Radiol)
Vol. 114
Issue 6
Pg. 771-7
(Dec 2012)
ISSN: 2212-4411 [Electronic] United States |
PMID | 23159115
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Elsevier Inc. All rights reserved. |
Chemical References |
- DNA Primers
- Wnt Proteins
- Alkaline Phosphatase
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Topics |
- Alkaline Phosphatase
(metabolism)
- Ameloblastoma
(genetics)
- Base Sequence
- Blotting, Western
- DNA Primers
- Down-Regulation
- Humans
- Immunohistochemistry
- Jaw Neoplasms
(genetics)
- Osteogenesis
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Wnt Proteins
(genetics)
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