An antioxidant regenerating system for continuous quenching of free radicals in chronic wounds.

Abstract

A novel antioxidant regenerating system consisting of cellobiose dehydrogenase (CDH), cellobiose, and phenolic antioxidants with potential application for continuous quenching of free radical species in chronic wounds was developed. This antioxidant regenerating system, continuously quenched in situ produced ·NO, O(2)(·-) and OH· radicals and the produced oxidized phenolic antioxidants were regenerated back to their original parent compounds by CDH using cellobiose as electron donor. This system therefore prevented the accumulation of oxidized phenolic antioxidants. Interestingly, this study also challenges the relevance of using total antioxidant capacities values of plant crude extracts obtained using biologically none relevant radical species like (2,2-diphenyl-1-picrylhydrazyl (DPPH)), Trolox Equivalent Antioxidant Capacity (TEAC), etc. when applied as medicinal remedies. This is because methoxylated phenolic antioxidants like sinapic acid, ferulic acid; 2,6-dimethoxyphenol readily donate their electrons to these radicals (DPPH, TEAC, etc.), thereby greatly influencing the total antioxidant values although this study showed that they are not at all effective in quenching O(2)(·-) radicals and again are not the most effective quenchers of NO and OH radicals as demonstrated during this study.

• Authors: Gibson S Nyanhongo, Christoph Sygmund, Roland Ludwig, Endry Nugroho Prasetyo, Georg M Guebitz
• Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (Eur J Pharm Biopharm) Vol. 83 Issue 3 Pg. 396-404 (Apr 2013) ISSN: 1873-3441 [Electronic] Netherlands
• PMID: 23153671 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Antioxidants
• Free Radicals
• Carbohydrate Dehydrogenases
• cellobiose-quinone oxidoreductase

Topics

• Antioxidants (pharmacology)
• Carbohydrate Dehydrogenases (metabolism)
• Chromatography, High Pressure Liquid
• Chronic Disease
• Free Radicals (metabolism)
• Mass Spectrometry
• Wounds and Injuries (metabolism)