Detection of cancer cells using a peptide nanotube-folic acid modified graphene electrode.
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| Abstract |
This article describes the preparation of a graphene electrode modified with a new conjugate of peptide nanotubes and folic acid for the selective detection of human cervical cancer cells over-expressing folate receptors. The functionalization of peptide nanotubes with folic acid was confirmed by fluorescence microscopy and atomic force microscopy. The peptide nanotube-folic acid modified graphene electrode was characterized by scanning electron microscopy and cyclic voltammetry. The modification of the graphene electrode with peptide nanotube-folic acid led to an increase in the current signal. The human cervical cancer cells were bound to the modified electrode through the folic acid-folate receptor interaction. Cyclic voltammograms in the presence of [Fe(CN)(6)](3-/4-) as a redox species demonstrated that the binding of the folate receptor from human cervical cancer cells to the peptide nanotube-folic acid modified electrode lowered the electron transfer resulting in a decrease in the measured current. A detection limit of 250 human cervical cancer cells per mL was obtained. Control experiments confirmed that the peptide nanotube-folic acid electrode specifically recognized folate receptors. The modified electrode described here opens up new possibilities for future applications in early stage diagnoses of diseases where cells over-express folate receptors, such as in cancer or leishmaniasis disease.
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| Authors | John J Castillo, Winnie E Svendsen, Noemi Rozlosnik, Patricia Escobar, Fernando Martínez, Jaime Castillo-León |
| Journal | The Analyst (Analyst) Vol. 138 Issue 4 Pg. 1026-31 (Feb 21 2013) ISSN: 1364-5528 [Electronic] England |
| PMID | 23150875 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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