Abstract |
In order to study the functions of a cell's endogenous mutant p53, the p53 protein levels must be knocked-down. Transient transfection of small interfering RNAs is one way to accomplish this. Another is the stable expression of short hairpin RNAs. This chapter presents a method by which a short hairpin RNA ( shRNA) targeting p53 is inserted into the genome of a cell via lentivirus infection. These p53 knock-down cell lines are stable and may be grown long term for use in a wide range of applications.
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Authors | Catherine Vaughan, Swati Palit Deb, Sumitra Deb |
Journal | Methods in molecular biology (Clifton, N.J.)
(Methods Mol Biol)
Vol. 962
Pg. 193-9
( 2013)
ISSN: 1940-6029 [Electronic] United States |
PMID | 23150448
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Viral
- Mutant Proteins
- RNA, Messenger
- RNA, Small Interfering
- Tumor Suppressor Protein p53
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Topics |
- DNA, Viral
(genetics)
- Gene Knockdown Techniques
(methods)
- Genetic Vectors
- HEK293 Cells
- Humans
- Lentivirus
- Mutant Proteins
(genetics)
- RNA, Messenger
(genetics)
- RNA, Small Interfering
(genetics)
- Transfection
- Tumor Suppressor Protein p53
(genetics)
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