Abstract | BACKGROUND: AIM: To examine the association between polβ polymorphism and ovarian cancer, case control study was performed using one hundred fifty two cancer samples and non-metastatic normal samples from the same patients in Indian population. DESIGN: The polβ polymorphism was studied in ovarian carcinoma tissues samples initially by RT-PCR followed by sequencing and then by western blot analysis. RESULT: A new type of variant was detected along with the WT allele (polβΔ<formula> _{208-304}</formula>). Stage IV samples have shown a significant factor for cancer progression in ovarian cancer patients of India [OR=3.58; 95% CI (1.6-7.9); and p=0.001]. The association study involving serous type and the variant showed a tendency towards ovarian carcinogenesis [OR=1.57; 95% CI (0.8-3.1); p=0.19]. The western blot analysis result indicates that the specific deletion appears to be associated with disease progression. CONCLUSION: The result reveals that this variant form of polβ is a predisposing factor for stage IV ovarian cancer samples in Indian population.
|
Authors | Kalyani Khanra, Kakali Panda, Chandan Bhattacharya, Ak Mitra, Ranu Sarkar, Sipra Banerjee, Nandan Bhattacharyya |
Journal | Cancer biomarkers : section A of Disease markers
(Cancer Biomark)
Vol. 11
Issue 4
Pg. 155-60
( 2012)
ISSN: 1875-8592 [Electronic] Netherlands |
PMID | 23144153
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
|
Topics |
- Adult
- Aged
- Alleles
- DNA Polymerase beta
(genetics, metabolism)
- DNA Repair
- Female
- Humans
- Middle Aged
- Neoplasm Staging
- Ovarian Neoplasms
(enzymology, genetics, pathology)
- Survival Analysis
- Young Adult
|