Abstract | BACKGROUND:
Ceramides are essential stratum corneum (SC) lipids and they play a pivotal role in maintaining effective cutaneous barrier function. OBJECTIVES: The present study aimed at determining the effect of a Dermatophagoides farinae house dust mite (Df-HDM) allergen challenge on SC ceramides of atopic dogs experimentally sensitized to these allergens. ANIMALS: Six Df-HDM-sensitized atopic Maltese-beagle dogs were used. METHODS: Prechallenge SC was obtained by cyanoacrylate stripping. One week later, the dogs were challenged topically with Df-HDM allergens, which resulted in mild to moderate inflammation 24 h later. Two weeks after challenge, SC of lesional and nonlesional skin was obtained. Finally, SC was collected from challenge sites 2 months after lesion resolution. The different SC lipids were quantified blindly by thin-layer chromatography. RESULTS: Significantly lower amounts of ceramides [AH], [AP], [AS], [NP], [EOP], [NS] and [EOS] were observed in lesional SC compared with prechallenge samples, while no significant effect was found on the amount of other lipids, including cholesterol and free fatty acids. The ceramide profile of nonlesional skin generally showed the same postchallenge reduction pattern. Ceramide amounts returned to normal within 2 months after lesion remission. CONCLUSION AND CLINICAL IMPORTANCE:
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Authors | Jessica Stahl, Judy Paps, Wolfgang Bäumer, Thierry Olivry |
Journal | Veterinary dermatology
(Vet Dermatol)
Vol. 23
Issue 6
Pg. 497-e97
(Dec 2012)
ISSN: 1365-3164 [Electronic] England |
PMID | 23140315
(Publication Type: Journal Article)
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Copyright | © 2012 The Authors. Veterinary Dermatology © 2012 ESVD and ACVD. |
Chemical References |
- Antigens, Dermatophagoides
- Ceramides
- Fatty Acids, Nonesterified
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Topics |
- Animals
- Antigens, Dermatophagoides
(toxicity)
- Ceramides
(chemistry, metabolism)
- Dermatitis, Atopic
(immunology, metabolism, pathology, veterinary)
- Dog Diseases
(immunology, metabolism, pathology)
- Dogs
- Epidermis
(metabolism)
- Fatty Acids, Nonesterified
(metabolism)
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