Attenuation of brain edema and spatial learning deficits by the inhibition of NADPH oxidase activity using apocynin following diffuse traumatic brain injury in rats.

Abstract

Diffuse brain injury (DBI) is a leading cause of mortality and disability among young individuals and adults worldwide. In specific cases, DBI is associated with permanent spatial learning dysfunction and motor deficits due to primary and secondary brain damage. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) is a major complex that produces reactive oxygen species (ROS) during the ischemic period. The complex aggravates brain damage and cell death following ischemia/reperfusion injury; however, its role in DBI remains unclear. The present study aimed to investigate the hypothesis that levels of NOX2 (a catalytic subunit of NOX) protein expression and the activation of NOX are enhanced following DBI induction in rats and are involved in aggravating secondary brain damage. A rat model of DBI was created using a modified weight-drop device. Our results demonstrated that NOX2 protein expression and NOX activity were enhanced in the CA1 subfield of the hippocampus at 48 and 72 h following DBI induction. Treatment with apocynin (50 mg/kg body weight), a specific inhibitor of NOX, injected intraperitoneally 30 min prior to DBI significantly attenuated NOX2 protein expression and NOX activation. Moreover, treatment with apocynin reduced brain edema and improved spatial learning function assessed using the Morris water maze. These results reveal that treatment with apocynin may provide a new neuroprotective therapeutic strategy against DBI by diminishing the upregulation of NOX2 protein and NOX activity.

• Authors: Si-Xin Song, Jun-Ling Gao, Kai-Jie Wang, Ran Li, Yan-Xia Tian, Jian-Qiang Wei, Jian-Zhong Cui
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 7 Issue 1 Pg. 327-31 (Jan 2013) ISSN: 1791-3004 [Electronic] Greece
• PMID: 23128834 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acetophenones
• Membrane Glycoproteins
• acetovanillone
• CYBB protein, human
• NADPH Oxidase 2
• NADPH Oxidases

Topics

• Acetophenones (administration & dosage, pharmacology)
• Animals
• Brain Edema (drug therapy, enzymology, etiology)
• Brain Injuries (complications)
• Disease Models, Animal
• Enzyme Activation (drug effects)
• Learning Disabilities (drug therapy, enzymology, etiology)
• Male
• Maze Learning (drug effects)
• Membrane Glycoproteins (metabolism)
• Memory (drug effects)
• NADPH Oxidase 2
• NADPH Oxidases (antagonists & inhibitors, metabolism)
• Rats