Evaluation of the role of CD207 on Langerhans cells in a murine model of atopic dermatitis by in situ imaging using Cr:forsterite laser-based multimodality nonlinear microscopy.

Abstract	Atopic dermatitis (AD) is an allergic inflammatory disease of skin. It remains unclear that CD207 of Langerhans cells (LCs) plays a central role in the development of allergic sensitization. There is little data on LCs within the microenviroment in vivo. We used a murine model of epicutaneous (EC) ovalbumin (OVA) sensitization inducing an inflammatory skin resembling AD to explore the role of CD207 in the pathogenesis of AD. Cr:forsterite laser-based multimodality nonlinear microscopy was applied for in situ imaging. Peritoneal injections of Alexa Fluor 647-rat anti-mouse CD207 into mice were performed to specifically trace the LCs. Peritoneal injections of OVA-Alexa Fluor 647 conjugate into mice were performed to specifically trace the OVA. We found that combining Alexa Fluor fluorescent probes with multimodality nonlinear microscopy permitted the unequivocal in situ imaging of CD207-expressing LCs. The relevant time-course, expressional, and functional studies reveal that CD207 of LCs plays an essential role during the induction of EC sensitization. We establish and validate that Cr:forsterite laser-based multimodality nonlinear microscopy is applicable for the specific detection of labeled mAb-bound LCs and labeled antigen. We suggest that CD207-expressing LCs initiate the allergic response through the CD207 mediated epicutaneous sensitization associated with the development of AD.
Authors	Jyh-Hong Lee, Ming-Rung Tsai, Chi-Kuang Sun, Bor-Luen Chiang
Journal	Journal of biomedical optics (J Biomed Opt) Vol. 17 Issue 11 Pg. 116007 (Nov 2012) ISSN: 1560-2281 [Electronic] United States
PMID	23117802 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Alexa Fluor 647 Antibodies, Monoclonal Antigens, Surface Carbocyanines Cd207 protein, mouse Fluorescent Dyes Lectins, C-Type Mannose-Binding Lectins Silicon Compounds forsterite Chromium Ovalbumin
Topics	Animals Antibodies, Monoclonal Antigens, Surface (metabolism) Carbocyanines Cell Count Chromium Dermatitis, Atopic (etiology, immunology, pathology) Female Fluorescent Dyes Immunization Langerhans Cells (immunology, pathology) Lectins, C-Type (metabolism) Mannose-Binding Lectins (metabolism) Mice Mice, Inbred BALB C Microscopy, Fluorescence, Multiphoton (methods) Optical Phenomena Ovalbumin (immunology) Rats Silicon Compounds Skin (cytology, immunology)

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